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  4. Chicken Cluster of Differentiation 4(CD4) ELISA Kit

Chicken Cluster of Differentiation 4(CD4) ELISA Kit

  • 中文名稱：
    雞CD4分子(CD4)酶聯免疫試劑盒
  • 貨號：
    CSB-E13114C
  • 規格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    雞CD4分子(CD4)酶聯免疫試劑盒（CSB-E13114C）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CD4含量。CD4是一種T細胞受體共受體，位于T細胞表面，與MHC II類分子結合，協助T細胞識別抗原。CD4在HIV感染中起關鍵作用，HIV病毒通過結合CD4進入T細胞，導致CD4+T細胞數量減少，免疫功能受損。研究CD4的功能和調控機制對于理解HIV感染和開發抗病毒藥物具有重要意義。試劑盒檢測范圍為3.125 ng/mL-800 ng/mL，適用于科研領域中對雞免疫系統的功能研究，包括感染性疾病模型中的T細胞應答分析、疫苗免疫效果評估以及免疫抑制性疾病機制探索等基礎研究，為禽類免疫學實驗提供可靠的定量檢測工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Gallus gallus (Chicken)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    3.125 ng/mL-800 ng/mL
  • 靈敏度：
    3.125 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of chicken CD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    84

    Range %

    80-89

    1:2

    Average %

    91

    Range %

    87-96

    1:4

    Average %

    92

    Range %

    87-98

    1:8

    Average %

    101

    Range %

    97-105

  • 回收率：

    The recovery of chicken CD4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    92

    88-96

    EDTA plasma (n=4)

    104

    101-107

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    800

    0.174

    0.172

    0.173

    200

    0.291

    0.275

    0.283

    50

    0.508

    0.546

    0.527

    12.5

    0.813

    0.835

    0.824

    3.125

    1.365

    1.398

    1.382

    0

    2.105

    2.168

    2.137

  • 數據處理：
  • 貨期：
    3-5 working days

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