多克隆抗體定制服務

多克隆抗體存在于免疫動物的血清中，可通過直接分離血清獲得。由于具有可識別多個表位、可引起凝集反應、沉淀反應等優點，被廣泛應用于免疫學診斷領域。比如經常用到的Western blotting實驗中的酶標二抗和ELISA實驗中的包被抗體。而且制備費用也較單克隆抗體低。

華美生物自主建立抗體研發生產平臺，可為您提供可靠的多克隆抗體定制服務，并有多種免疫原和多種宿主物種可供選擇。您可以選擇自己提供抗原或者我們幫您設計并制備抗原，我們將提供給您滿足您實驗需求的多克隆抗體產品。

服務流程：

服務內容及周期：

免疫原	服務內容	交付內容	服務周期
多肽	抗原制備動物免疫血清滴度檢測親和純化或Protein A/G純化抗原 WB 驗證	提供抗原QC報告； ELISA滴度效價保證1:64000；抗原WB檢測結果保證陽性；提供1mL免疫前血清，2mL抗血清，5-10mg經protein A/G或親和純化的抗體；提供SDS-PAGE檢測結果，抗體純度保證90%。	12-14 周
重組蛋白			14-16 周
天然蛋白			12-14 周

服務實例：

ELISA
Antigen coating concentration 2 ug/ml
Antiserum 1:2000 is more than diluted
Secondary：Goat polyclonal to rabbit IgG at 1/50000 dilution

Western Blot
Positive WB detected in：K562 whole cell lysate,HL-60 whole cell lysate,Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate,Rat liver tissue, Mouse brain tissue, Mouse lung tissue
All lanes：a antibody at 2ug/ ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 64 KDa
Observed band size: 64 KDa

Western Blot
WT: Wild-type 293 cells
KO: Knockout 293 cells

IHC image of A antibody diluted at 1:400 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with A antibody at 1:400,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).