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Dog interleukin 12 (IL-12/P70) ELISA kit

  • 中文名稱:
    犬白介素12(IL-12/P70)酶聯免疫試劑盒
  • 貨號:
    CSB-EQ027628DO
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    犬白介素12(IL-12/P70)酶聯免疫試劑盒(CSB-EQ027628DO)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的IL12/P70含量。IL12/P70是一種重要的靶點。它在免疫調節中起關鍵作用,能促進Th1細胞分化、增強NK細胞活性等。研究主要圍繞其參與的免疫信號通路,探索通過調控它來治療如自身免疫性疾病、腫瘤等,有望為相關疾病治療帶來新策略。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL,適用于犬類免疫機制研究,包括炎癥性疾病模型建立、疫苗免疫效果評估或感染后細胞因子動態監測等科研領域,可為動物免疫學、傳染病學及比較醫學研究提供可靠的實驗數據支持。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    IL12/P70, Interleukin-12/P70
  • 縮寫:
    IL-12/P70
  • 種屬:
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍:
    15.6 pg/mL-1000 pg/mL
  • 靈敏度:
    3.9 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %95
    Range %91-105
    1:2Average %87
    Range %84-93
    1:4Average %97
    Range %91-106
    1:8Average %94
    Range %86-98
  • 回收率:
    The recovery of dog IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9087-96
    EDTA plasma (n=4)9792-106
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.912 2.945 2.929 2.829
    5002.546 2.578 2.562 2.462
    2501.780 1.806 1.793 1.693
    1251.127 1.226 1.177 1.077
    62.50.546 0.576 0.561 0.461
    31.20.259 0.269 0.264 0.164
    15.60.168 0.176 0.172 0.072
    00.095 0.105 0.100  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價



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