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Fish Complement 3,C3 ELISA Kit

  • 中文名稱:
    魚補體蛋白3(C3)酶聯免疫試劑盒
  • 貨號:
    CSB-E09727s
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    12.5 μg/mL-500 μg/mL
  • 靈敏度:
    12.5 μg/mL
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    魚補體蛋白3(C3)酶聯免疫試劑盒(CSB-E09727s)為競爭法ELISA試劑盒,定量檢測血清、血漿樣本中的C3含量。C3是補體系統重要成分。其背景在于參與免疫防御、免疫自穩等。研究機制方面,它可被多種途徑激活,裂解為多個片段,參與調理吞噬、介導炎癥反應等,在抗感染、自身免疫病等研究中有重要價值。試劑盒檢測范圍為12.5 μg/mL-500 μg/mL,該產品廣泛應用于水產動物免疫機制研究,如細菌或病毒感染模型中的補體通路分析、養殖環境壓力對免疫功能的評估,以及疫苗或免疫增強劑的功效驗證等實驗場景,為水生生物健康管理和疾病防控研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
  • 種屬:
    Fish
  • 反應時間:
    1-5h
  • 上樣量:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cancer
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of fish C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 85-100  
    1:2 Average % 96  
    Range % 90-108  
    1:4 Average % 95  
    Range % 91-98  
    1:8 Average % 95  
    Range % 91-100  
  • 回收率:
    The recovery of fish C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 89 85-94  
    EDTA plasma (n=4) 95 91-102  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    500 0.176 0.174 0.175    
    250 0.381 0.393 0.387    
    115 0.557 0.569 0.563    
    37.5 0.894 0.901 0.898    
    12.5 1.481 1.498 1.490    
    0 2.417 2.542 2.480    
  • 數據處理:
  • 貨期:
    3-5 working days

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