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Fish Insulin,INS ELISA Kit

  • 中文名稱:
    魚胰島素(INS)酶聯免疫試劑盒
  • 貨號:
    CSB-E12123Fh
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    魚胰島素(INS)酶聯免疫試劑盒(CSB-E12123Fh)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的INS含量。INS即胰島素基因,其表達產生的胰島素在調節血糖穩態中至關重要。研究機制方面,圍繞胰島素的分泌、作用信號通路等展開,異常的INS表達或功能與糖尿病等代謝疾病密切相關,對其深入研究有助于揭示疾病發病機制及開發新療法。試劑盒檢測范圍為100 pg/mL-1600 pg/mL,該產品適用于魚類生理學研究,例如評估不同生長階段、營養狀態或環境脅迫(如水溫、鹽度變化)對胰島素分泌的影響,也可用于水產養殖中魚類代謝疾病模型的機制探索。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Fish
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    100 pg/mL-1600 pg/mL
  • 靈敏度:
    100 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Metabolism
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of fish INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 86-100  
    1:2 Average % 98  
    Range % 92-103  
    1:4 Average % 85  
    Range % 80-89  
    1:8 Average % 91  
    Range % 83-99  
  • 回收率:
    The recovery of fish INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 88-98  
    EDTA plasma (n=4) 92 90-95  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    1600 0.178 0.189 0.184    
    800 0.322 0.340 0.331    
    400 0.610 0.634 0.622    
    200 1.257 1.230 1.244    
    100 2.335 2.475 2.405    
  • 數據處理:
  • 貨期:
    3-5 working days

引用文獻

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相關問答

 常見問題解答
Q:

我想找一款可以檢測斑馬魚胰島素水平的ELISA試劑盒。你有推薦的么?

A:
我們有以下魚試劑盒: #CSB-E12123Fh,魚胰島素,INS ELISA Kit 一般來說,同一物種間的蛋白質差異不大。也就是說,不同類型魚類的氨基酸序列相對保守 這個項目#CSB-E12123Fh是專門針對鮭魚的。我們沒有測試過斑馬魚。但應該沒有什么大問題,你可以試著先做一個預實驗。