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  4. Fish metallothionein(MT)ELISA kit

Fish metallothionein(MT)ELISA kit

  • 中文名稱：
    魚金屬硫蛋白(MT)酶聯免疫試劑盒
  • 貨號：
    CSB-EQ027262FI
  • 規格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    魚金屬硫蛋白(MT)酶聯免疫試劑盒（CSB-EQ027262FI）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的MT含量。試劑盒檢測范圍為25 pg/mL-400 pg/mL，適用于水產養殖研究、水生態系統重金屬污染監測、魚類生理應激機制分析等科研領域。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    MT
  • 種屬：
    Fish
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    25 pg/mL-400 pg/mL
  • 靈敏度：
    12.5 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Epigenetics and Nuclear Signaling
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish MT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 84  
    Range % 80-88  
    1:200 Average % 90  
    Range % 86-94  
    1:400 Average % 99  
    Range % 94-103  
    1:800 Average % 92  
    Range % 88-96  
  • 回收率：
    The recovery of fish MT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 92-102  
    EDTA plasma (n=4) 95 89-99  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    400 0.103 0.100 0.102    
    200 0.247 0.228 0.238    
    100 0.401 0.387 0.394    
    50 0.646 0.598 0.622    
    25 0.983 0.966 0.975    
    0 1.572 1.529 1.551    
  • 數據處理：
  • 貨期：
    3-5 working days

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