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Human Coll2-1 ELISA kit

  • 中文名稱:
    人Coll2-1酶聯免疫試劑盒
  • 貨號:
    CSB-EQ027311HU
  • 樣本類型:
    serum, plasma, cell culture supernates
  • 檢測范圍:
    47 pg/mL-3000 pg/mL
  • 靈敏度:
    11.75 pg/mL
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    人Coll2-1酶聯免疫試劑盒(CSB-EQ027311HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織培養上清液樣本中的Coll2-1含量。試劑盒檢測范圍為47 pg/mL-3000 pg/mL,Coll2-1是關節軟骨中II型膠原蛋白特異性降解的生物標志物,其水平變化與關節退行性疾病及軟骨代謝異常密切相關,在骨關節炎機制研究、藥物開發療效評估及運動醫學相關科研領域具有重要應用價值。試劑盒包含預包被抗體、標準品及檢測所需試劑,操作流程標準化,適用于體外檢測科研樣本中Coll2-1的動態變化,可為軟骨代謝研究、疾病模型建立及生物標志物篩選提供可靠數據支持。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    Coll2-1
  • 種屬:
    Homo sapiens (Human)
  • 反應時間:
    1-5h
  • 上樣量:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human Coll2-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:100Average %85
    Range %80-90
    1:200Average %98
    Range %90-105
    1:400Average %95
    Range %90-100
    1:800Average %92
    Range %87-97
  • 回收率:
    The recovery of human Coll2-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 89 83-95  
    EDTA plasma (n=4) 98 92-105  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    3000 2.513 2.487 2.500 2.417  
    1500 1.847 1.794 1.821 1.738  
    750 1.198 1.112 1.155 1.072  
    375 0.754 0.709 0.732 0.649  
    187.5 0.351 0.337 0.344 0.261  
    94 0.198 0.183 0.191 0.108  
    47 0.134 0.125 0.130 0.047  
    0 0.084 0.081 0.083    
  • 數據處理:
  • 貨期:
    3-5 working days

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