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  4. Human Epinephrine/Adrenaline,EPI ELISA Kit

Human Epinephrine/Adrenaline,EPI ELISA Kit

  • 中文名稱：
    人腎上腺素(EPI)酶聯免疫試劑盒
  • 貨號：
    CSB-E08677h
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    人腎上腺素(EPI)酶聯免疫試劑盒（CSB-E08677h）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的EPI含量。試劑盒檢測范圍為2 pg/mL-400 pg/mL，適用于神經生物學、內分泌學及藥理學等領域的基礎研究，例如應激狀態下激素分泌機制探索、心血管疾病模型中的激素動態監測，或藥物干預對腎上腺功能影響的評價等科研場景本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    EPI
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    2 pg/mL-400 pg/mL
  • 靈敏度：
    1 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human EPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 87-98  
    1:2 Average % 95  
    Range % 90-99  
    1:4 Average % 90  
    Range % 85-96  
    1:8 Average % 90  
    Range % 84-98  
  • 回收率：
    The recovery of human EPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 87 80-94  
    EDTA plasma (n=4) 98 92-107  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    400 0.213 0.210 0.212    
    100 0.408 0.391 0.400    
    32 0.613 0.631 0.622    
    8 1.062 1.003 1.033    
    2 1.593 1.516 1.555    
    0 2.467 2.412 2.440    
  • 數據處理：
  • 貨期：
    3-5 working days

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