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Human F-actin ELISA Kit

  • 中文名稱:
    人F-肌動蛋白酶聯免疫試劑盒
  • 貨號:
    CSB-E13678h
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    人F-肌動蛋白酶聯免疫試劑盒(CSB-E13678h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的F-actin含量。F-actin即絲狀肌動蛋白,是細胞骨架的重要組成部分。其在細胞形態維持、運動遷移、物質運輸等過程中發揮關鍵作用。研究機制上,主要聚焦于其聚合和解聚動態平衡調控,以及與多種結合蛋白相互作用,影響細胞生理和病理狀態。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL,適用于細胞骨架動態研究、細胞力學特性分析、心血管疾病或腫瘤轉移機制探索等科研領域,尤其可為細胞應激反應、病理模型構建及藥物干預效果評估提供可靠的分子檢測工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    F-actin
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.625 ng/mL-40 ng/mL
  • 靈敏度:
    0.156 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human F-actin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %91
    Range %86-95
    1:2Average %102
    Range %97-107
    1:4Average %91
    Range %85-97
    1:8Average %97
    Range %91-103
  • 回收率:
    The recovery of human F-actin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9591-101
    EDTA plasma (n=4)8885-96
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    402.691 2.607 2.649 2.430
    202.304 2.139 2.222 2.003
    101.804 1.862 1.833 1.614
    51.319 1.331 1.325 1.106
    2.50.854 0.907 0.881 0.662
    1.250.592 0.549 0.571 0.352
    0.6250.385 0.367 0.376 0.157
    00.226 0.212 0.219  
  • 數據處理:
  • 貨期:
    3-5 working days

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