Human Hyperglycosylated Chorionic Gonadotropin(hCG) ELISA Kit

產品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人高糖基化人絨毛膜促性腺激素(hCG)酶聯免疫試劑盒（CSB-E15803h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、尿液、組織培養上清液樣本中的hCG含量。hCG即人絨毛膜促性腺激素，是妊娠時胎盤滋養層細胞分泌的糖蛋白。在醫學研究中，其可用于妊娠診斷。研究機制上，它能促進黃體發育，維持妊娠狀態，還和腫瘤發生發展相關，在腫瘤診斷和治療靶點研究受關注。試劑盒檢測范圍為0.67 mIU/mL-20 mIU/mL，該產品適用于生殖生物學研究，如妊娠相關分子機制探索、滋養層細胞功能分析，也可用于腫瘤生物學領域中對hCG異常表達的機制研究，或體外細胞模型中激素分泌水平的動態監測。通過標準化的實驗流程，為科研工作者提供穩定可靠的數據支持，滿足生殖醫學、細胞生物學及疾病模型構建等領域的高靈敏度檢測需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

N/A
縮寫：

hCG
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, urine, cell culture supernates
檢測范圍：

0.67 mIU/mL-20 mIU/mL
靈敏度：

0.167 mIU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human hCG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-99
1:2	Average %	97
	Range %	92-103
1:4	Average %	96
	Range %	90-100
1:8	Average %	95
	Range %	89-101

回收率：

The recovery of human hCG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	91-103
EDTA plasma (n=4)	94	89-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.