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  4. Human Interleukin 8,IL-8 ELISA KIT
  • 中文名稱：
    人白介素8(IL-8/CXCL8)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E04641h
  • 樣本類型：
    serum, cell culture supernates, saliva, urine, cerebrospinal fluid (CSF), tissue homogenates, cell lysates
  • 檢測范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.110 pg/mL
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人白介素8(IL-8/CXCL8)酶聯(lián)免疫試劑盒（CSB-E04641h）為雙抗夾心法ELISA試劑盒，定量檢測血清、組織培養(yǎng)上清液、唾液、尿液、腦脊液（CSF）、組織勻漿、細(xì)胞裂解物樣本中的CXCL8含量。CXCL8，又稱白細(xì)胞介素-8或CXCL8，是一種促炎CXC趨化因子，主要由免疫細(xì)胞和腫瘤細(xì)胞分泌。它通過兩種受體CXCR1和CXCR2作用于人類中性粒細(xì)胞，參與炎癥反應(yīng)和腫瘤發(fā)生發(fā)展。CXCL8的研究機(jī)制涉及信號(hào)通路、轉(zhuǎn)錄因子調(diào)控以及與腫瘤細(xì)胞、血管生成等生物學(xué)過程的關(guān)系。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，靈敏度為7.110 pg/mL。適用于炎癥機(jī)制研究、藥物作用評(píng)價(jià)、疾病模型構(gòu)建等科研場景，助力免疫調(diào)控網(wǎng)絡(luò)及細(xì)胞信號(hào)通路的深入探索，滿足體外實(shí)驗(yàn)中IL - 8蛋白表達(dá)量監(jiān)測需求本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    (Ala-IL-8)77 ELISA Kit; (Ser-IL-8)72 ELISA Kit; 9E3 ELISA Kit; Beta thromboglobulin like protein ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; CEF-4 ELISA Kit; chemokine; CXC motif; ligand 8 ELISA Kit; CXCL8 ELISA Kit; Emoctakin ELISA Kit; GCP-1 ELISA Kit; GCP/IL-8 protein I ELISA Kit; GCP/IL-8 protein II ELISA Kit; GCP/IL-8 protein III ELISA Kit; GCP/IL-8 protein IV ELISA Kit; GCP/IL-8 protein V ELISA Kit; GCP/IL-8 protein VI ELISA Kit; GCP1 ELISA Kit; Granulocyte chemotactic protein 1 ELISA Kit; IL-8 ELISA Kit; IL-8(1-77) ELISA Kit; IL-8(9-77) ELISA Kit; IL8 ELISA Kit; IL8/NAP1 form I ELISA Kit; IL8/NAP1 form II ELISA Kit; IL8/NAP1 form III ELISA Kit; IL8/NAP1 form IV ELISA Kit; IL8/NAP1 form V ELISA Kit; IL8/NAP1 form VI ELISA Kit; IL8_HUMAN ELISA Kit; Inteleukin 8 ELISA Kit; LECT ELISA Kit; LUCT ELISA Kit; Lymphocyte-derived neutrophil-activating factor ELISA Kit; LYNAP ELISA Kit; MDNCF ELISA Kit; MDNCF-b ELISA Kit; MDNCF-c ELISA Kit; MONAP ELISA Kit; Monocyte derived neutrophil activating peptide ELISA Kit; Monocyte derived neutrophil chemotactic factor ELISA Kit; Monocyte-derived neutrophil chemotactic factor ELISA Kit; Monocyte-derived neutrophil-activating peptide ELISA Kit; NAF ELISA Kit; NAP 1 ELISA Kit; NAP-1 ELISA Kit; NAP1 ELISA Kit; Neutrophil activating peptide 1 ELISA Kit; Neutrophil activating protein 1 ELISA Kit; Neutrophil-activating factor ELISA Kit; Neutrophil-activating protein 1 ELISA Kit; Protein 3 10C ELISA Kit; Protein 3-10C ELISA Kit; SCYB 8 ELISA Kit; SCYB8 ELISA Kit; Small inducible cytokine subfamily B member 8 ELISA Kit; T cell chemotactic factor ELISA Kit; T-cell chemotactic factor ELISA Kit; TSG 1 ELISA Kit; TSG1 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 反應(yīng)時(shí)間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

     Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess. 

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

  • 本試劑盒所含材料：
      • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human IL-8 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labled IL-8 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Squirt bottle, manifold dispenser, or automated microplate washer.
      • Absorbent paper for blotting the microtiter plate.
      • 100ml and 500ml graduated cylinders.
      • Deionized or distilled water.
      • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL and pipette tips.
      • Timer
      • Test tubes for dilution.

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 1 個(gè)評(píng)價(jià)

樣品類型：細(xì)胞培養(yǎng)上清液

樣品信息：人

稀釋比：其他 1:10

產(chǎn)品評(píng)價(jià)： 人細(xì)胞系檢測培養(yǎng)基上清IL-8, 對(duì)照組OD平均約0.7，模型組平均1.5，是想要的趨勢，操作簡單，靈敏度高，推薦

By 王老師

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
    IL-8，也稱為促炎細(xì)胞因子（CXCL8），是一種由多種細(xì)胞類型產(chǎn)生的趨化因子，可誘導(dǎo)白細(xì)胞浸潤到炎癥部位并發(fā)揮其炎癥介導(dǎo)功能。近年來的研究發(fā)現(xiàn)，IL-8不僅在炎癥反應(yīng)中發(fā)揮作用，還參與了許多疾病的發(fā)生和發(fā)展，如腫瘤、心血管疾病、感染性疾病等。因此，IL-8已成為炎癥、免疫和疾病研究領(lǐng)域的熱門研究對(duì)象。目前，研究人員正在探索使用IL-8作為治療靶點(diǎn)的潛力，并且已經(jīng)有一些前期結(jié)果表明，針對(duì)IL-8的治療可能會(huì)在一些疾病中具有顯著的治療效果。
  • 功能：
    IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
  • 基因功能參考文獻(xiàn)：
    1. VEGF and IL-8 play a prominent role in the pathogenesis of early forms of rosacea and the hemostasis system. PMID: 29578433
    2. High levels of LIFR in colorectal cancer (CRC) facilitated proliferation and migration of endothelial cells, resulting in an increase in angiogenic activity. Moreover, IL-8 was found to play a role in the LIFR induced angiogenesis. IL-8 levels were correlated with LIFR levels in CRC tissues, whereas depletion of IL-8 led to a reduced angiogenic activity of LIFR in CRC cells. PMID: 29751081
    3. PKC-delta isoform plays a crucial role in Tat-TLR4 signaling pathway to activate NF-kappaB and CXCL8 production. PMID: 28539656
    4. CXCL8 was a target of miR-204, and miR-204 suppression could not increase cell viability, migration, invasion, and EMT procedure when CXCL8 was silenced. PMID: 29402343
    5. Interleukin 8 - 845 T/C and + 781 C/T polymorphisms were analyzed. For + 781C/T locus, in the dominant genetic model, significant difference between TT vs. CC + CT genotypes that significantly had a protective role against periodontitis disease. Positive association between distribution of IL8 - 845 T/C alleles and risk of periodontitis disease. C allele of IL-8 - 845 increased the risk of periodontitis disease. PMID: 30078118
    6. These results suggest a direct involvement of IL-8-CXCR1/2 axes in GBM progression by promoting both cell proliferation and invasion and indirectly by promoting neovascularization in the form of vascular mimicry. PMID: 30086759
    7. These results show that AKIP1 is crucial in cervical cancer angiogenesis and growth by elevating the levels of the NF-kappaB-dependent chemokines CXCL1, CXCL2, and CXCL8. PMID: 29520695
    8. The extramembranous domain of HofQ (emHofQ) was shown to interact with various cytokines, of which IL-8 exhibited the strongest interaction. PMID: 30088437
    9. he protein expression levels of IL-8 were significantly decreased in SZ patients, but no significant difference in the mRNA levels of IL-8 was observed between SZ patients and NC subjects. PMID: 28476335
    10. immune system process is indispensable in the progression of disease in colon, and identifies that IL-8 and MMP-9 play potential critical roles for the progression. PMID: 30074183
    11. IL-8 production was significantly enhanced following treatment with both IL-17A and CSE, while treatment with either IL-17A or CSE alone caused only a slight increase in IL-8 production. PMID: 29463070
    12. Work identified IL-8 as a positive regulator of homotypic CIC formation via enhancing intercellular adhesion. PMID: 30021676
    13. V2O5 induction of CXCL8 and CXCL11 chemokines may lead to the appearance and perpetuation of an inflammatory reaction into the dermal tissue. Further studies are required to evaluate dermal integrity and manifestations in subjects occupationally exposed, or living in polluted areas. PMID: 29901202
    14. Study findings point out that PAR2 could play an essential role in gastroesophageal reflux disease (GERD) pathogenesis - even repeated short-term exposure to weakly acidic conditions lead to the upregulation of PAR2 and subsequent activation of the intense IL-8 release in the esophageal mucosa and initiation of mucosal immune response in GERD. PMID: 29672302
    15. Given that IL-8, MIP-1beta, and MCP-1 are chemokines that play important roles in recruitment of immunocompetent cells for immune defense and tumor cell clearance, the observed lower levels of these markers with increasing PM2.5 exposure may provide insight into the mechanism by which DEE promotes lung cancer. PMID: 29023999
    16. These results suggested that stemness induction in SKOV3 cells by macrophages co-cultured with SKOV3-derived OCSLCs involved IL-8/STAT3 signaling. PMID: 29656182
    17. IL-8-251T>A (rs4073) Polymorphism is associated with gastric Cancer. PMID: 30275190
    18. expression level of CXCL8 had a positive relationship with recurrence probability in Acute myeloid leukemia. PMID: 29596823
    19. These data were in close agreement with the reduced cell migration and colony formation. Results from the present study suggested that reparixin and SCH527123 may be promising therapeutic agents for the treatment of pancreatic cancer by inhibiting the IL8/CXCR1/2 signaling cascade. PMID: 29749433
    20. A urinary IL-8 level of less than 61.25 pg/ml is more sensitive for prediction of complete remission in idiopathic membranous nephropathy patients. PMID: 29415357
    21. berberine inhibited the expression of MCP-1 and IL-8 induced by LPS. PMID: 28852897
    22. Regarding the IL-8 promoter T - 251A, the TA and AA genotypes were associated with significantly decreased risks of nasopharyngeal carcinoma (NPC) in a Taiwanese population compared with the wild-type TT genotype. The mRNA and protein expression levels for NPC tissues revealed no significant associations among the 20 NPC samples with different genotypes. PMID: 30200105
    23. IL-8 +781 T/C polymorphism is associated with the severe Clostridium difficile infection PMID: 29203364
    24. ShRNA mediated down-regulation of CXCL8 resulted in inhibition of cell proliferation, viability and invasion in vitro and a near complete growth reduction of tumor in vivo. PMID: 29679563
    25. CSF IL-8 concentrations were significantly elevated in CNS tumor patients as compared to non-tumoral individuals. AUC for CSF IL-8 was higher than for its index (CSF IL-8/serum IL-8). PMID: 29086194
    26. High IL8 expression is associated with melanoma. PMID: 29286146
    27. Lipo-CPFX, but not CPFX, retained the anti-IL-8 releasing activity. PMID: 29337216
    28. The results indicate significant contribution of IL8 on survival of hormonal dependent early-stage breast cancer patients and association with established parameters such as estrogen receptors/progesterone receptor and HER2. PMID: 28569250
    29. the frequency of non-classical monocytes expressing CXCL8 was increased in systemic sclerosis patient and monocytes expressing CXCL8 PMID: 29127442
    30. ntermediate Molecular Mass Hyaluronan and CD44 interactions enhanced normal PMN phagocytosis and IL-8 production PMID: 28730511
    31. serum levels in active vitiligo significantly elevated compared to those in stable vitiligo patients PMID: 29115683
    32. High IL8 expression is associated with pancreatic adenocarcinoma. PMID: 29205349
    33. Compared with controls, the interleukin (IL)-8 A/A genotype was more common in acute pancreatitis (AP). PMID: 29215544
    34. The presence of neither the first transmembrane helix of the receptor nor the lipid bilayer significantly affected the interactions of IL-8 with Binding Site-I of CXCR1. PMID: 29143165
    35. CXCL8 is highly expressed in cervical cancer tissues and cell lines, and correlated with malignant status and prognosis in cervical cancer patients. PMID: 28883082
    36. In conclusion, to our knowledge, this is the first study in the association of rs4073 and rs2227306 polymorphisms with childhood asthma risk in the Tunisian population. PMID: 28993876
    37. Results show that IL8 expression level is regulated by APE1 which activates NF-KB. PMID: 27388124
    38. aberrant miR-520c-3p expression may lead to reduced IL-8 expression and promote the mesenchymal phenotype in breast cancer cells, thereby increasing invasive growth. PMID: 29048659
    39. increased levels of IL-8 are associated with factors of worse prognosis in ovarian cancer PMID: 28872976
    40. Significantly elevated blood levels of IL-8 in myelodysplastic syndrome patients. PMID: 28856536
    41. the elevated concentrations of CXCL13, CXCL8, and CXCL10 or their increasing CSF/serum ratios may be potential biomarkers of neurosyphilis PMID: 27650493
    42. Results implicated the important role of PRL-3 in glycolysis metabolism through improving IL-8 secretion in colorectal cancer cells, and PRL-3 mediated glycolysis contributed to the promotion of cancer metastasis. PMID: 28791350
    43. Changes in serum IL-8 levels could be used to monitor and predict clinical benefit from immune checkpoint blockade in melanoma and NSCLC patients PMID: 28595336
    44. Lung cancer patients showed significantly lower levels of serum VEGF (1.9 fold) and IL-8 (~9 fold) than COPD patients. VEGF level was significantly higher (2.6 fold) in metastatic than non-metastatic cancer patients. An increase in MMP-9 (~1.6 fold) levels was observed in lung cancer patients. PMID: 27811960
    45. CXCL1/8 secreted by adipose-derived mesenchymal stem cells could promote breast cancer angiogenesis. PMID: 28514506
    46. our meta-analysis suggests that the IL-8 rs4073, A2767T, T11722T2, rs2234671, rs2230054, rs1126579, rs2227306, rs2227307, rs2227532, and T-738A polymorphisms are not associated with periodontitis while the IL-8 C1633T and rs1126580 polymorphisms may elevate the susceptibility of periodontitis based on the currently available evidences. PMID: 28446725
    47. An analogue of human CXCL8, CXCL8(3-72)K11R/G31P (hG31P) has been developed. PMID: 28754019
    48. inflammation triggered property of Microcystin-LR via IL-8/CXCR2 signaling PMID: 29197248
    49. A high IL-8 content in urine sampled on day 1 after renal transplantation was positively correlated with the activity of metalloproteinase-9 in urine. This proves that both of these chemokines cooperate in ischaemia-reperfusion injuries in transplanted kidneys. PMID: 28494217
    50. We discovered that IL-8 secreted from decidual stromal cells is a key cytokine enhancing the invasiveness of trophoblasts PMID: 28328096

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine alpha (chemokine CxC) family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 6025

    OMIM: 146930

    KEGG: hsa:3576

    STRING: 9606.ENSP00000306512

    UniGene: Hs.624