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Human S100 calcium-binding protein A8/A9 complex(S100A8/A9)Elisa kit

  • 中文名稱:
    人S100鈣結(jié)合蛋白A8/A9復(fù)合物(S100A8/A9)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E12149h
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人S100鈣結(jié)合蛋白A8/A9復(fù)合物(S100A8/A9)酶聯(lián)免疫試劑盒(CSB-E12149h)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的S100A8/A9含量。S100A8/A9是鈣結(jié)合蛋白S100家族成員,參與炎癥反應(yīng)和細(xì)胞生長(zhǎng)分化。研究發(fā)現(xiàn),S100A8/A9在多種腫瘤中高表達(dá),可能與腫瘤的發(fā)生發(fā)展密切相關(guān),其具體機(jī)制尚需進(jìn)一步研究。試劑盒檢測(cè)范圍為1.56 ng/mL-100 ng/mL,該產(chǎn)品適用于體外研究,如炎癥機(jī)制探索、疾病模型構(gòu)建、藥物干預(yù)效果評(píng)估或生物標(biāo)志物相關(guān)性分析,為研究感染性病變、腫瘤進(jìn)展、代謝綜合征等病理過程中 S100A8/A9 的動(dòng)態(tài)變化提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    N/A
  • 縮寫:
    S100A8/A9
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    1.56 ng/mL-100 ng/mL
  • 靈敏度:
    0.39 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Others
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human S100A8/A9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %87
    Range %80-92
    1:2Average %95
    Range %91-98
    1:4Average %92
    Range %87-98
    1:8Average %95
    Range %90-103
  • 回收率:
    The recovery of human S100A8/A9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8581-89
    EDTA plasma (n=4)9285-96
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    1002.545 2.541 2.543 2.461
    501.850 1.726 1.788 1.706
    251.021 1.001 1.011 0.929
    12.50.583 0.567 0.575 0.493
    6.250.357 0.334 0.346 0.264
    3.120.205 0.198 0.202 0.120
    1.560.139 0.133 0.136 0.054
    00.085 0.079 0.082  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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