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  4. Human calcitonin gene related peptide,CGRP ELISA Kit

Human calcitonin gene related peptide,CGRP ELISA Kit

  • 中文名稱：
    人降鈣素基因相關肽(CGRP)酶聯免疫試劑盒
  • 貨號：
    CSB-E08210h
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    人降鈣素基因相關肽(CGRP)酶聯免疫試劑盒（CSB-E08210h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的CGRP含量。CGRP（降鈣素基因相關肽）是一種神經肽。在它在偏頭痛等疾病發病機制中扮演重要角色。研究機制上，它能擴張腦血管、調節神經炎癥。針對 CGRP 及其受體開發藥物以阻斷其作用，成為偏頭痛等治療的新策略。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL，適用于體外研究CGRP在神經生物學、心血管功能、疼痛機制或炎癥模型中的動態變化，例如評估實驗動物模型中CGRP的釋放水平、細胞因子刺激下培養細胞的分泌特征，或藥物干預對組織內CGRP表達的影響等科研用途本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Beta type CGRP ELISA Kit; Beta-CGRP ELISA Kit; Beta-type CGRP ELISA Kit; CALC2 ELISA Kit; Calcb ELISA Kit; CALCB_HUMAN ELISA Kit; Calcitonin gene related peptide 2 ELISA Kit; Calcitonin gene related peptide II ELISA Kit; Calcitonin gene-related peptide 2 ELISA Kit; Calcitonin gene-related peptide II ELISA Kit; Calcitonin related polypeptide; beta ELISA Kit; CGRP II ELISA Kit; CGRP-II ELISA Kit; CGRP2 ELISA Kit; CGRPII ELISA Kit; FLJ30166 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    1.56 pg/mL-100 pg/mL
  • 靈敏度：
    0.39 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %85
    Range %80-92
    1:2Average %96
    Range %91-105
    1:4Average %97
    Range %92-110
    1:8Average %90
    Range %86-98
  • 回收率：
    The recovery of human CGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)9690-100
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.660 2.511 2.586 2.432
    502.080 2.152 2.116 1.962
    251.451 1.366 1.409 1.255
    12.50.795 0.744 0.770 0.616
    6.250.491 0.523 0.507 0.353
    3.120.315 0.325 0.320 0.166
    1.560.248 0.244 0.246 0.092
    00.157 0.151 0.154 　
  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

相關問答

■ 常見問題解答
Q:

關于人降鈣素基因相關肽CGRP ELISA試劑盒（CSB-E08210h），這個CUSABIO的CGRP Sandwich ELISA檢測的具體是什么？全長還是片段？這個試驗能否檢測其他CGRP相關肽？

A:
CSB-E08210h 檢測的是Beta-type CGRP，參考https://www.uniprot.org/uniprot/P10092針對全長設計，至于客戶提到的other CGRP related peptides理論是檢測不了的。如果您有具體信息，可以發送給我們進一步嘗試。

靶點詳情

  • 功能：
    CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role.
  • 基因功能參考文獻：
    1. The results suggest that C12orf39, CSTA, and CALCB are novel ATF4 target genes, and that C12orf39 promoter activity is activated by ATF4 through amino acid response element. PMID: 26967115
    2. Keratinocyte-derived CGRPbeta may modulate epidermal homeostasis through autocrine/paracrine signaling and may contribute to chronic pain under pathological conditions. PMID: 21641113
    3. Calcitonin gene-related peptide stimulates proliferation of alveolar epithelial cells. PMID: 19192276
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Calcitonin family
  • 數據庫鏈接：

    HGNC: 1438

    OMIM: 114160

    KEGG: hsa:797

    STRING: 9606.ENSP00000346017

    UniGene: Hs.534305