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  4. Human hydroxyproline,Hyp ELISA Kit

Human hydroxyproline,Hyp ELISA Kit

  • 中文名稱：
    人羥脯氨酸(Hyp)酶聯免疫試劑盒
  • 貨號：
    CSB-E08837h
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    78 ng/mL-5000 ng/mL
  • 靈敏度：
    19.5 ng/mL
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    人羥脯氨酸(Hyp)酶聯免疫試劑盒（CSB-E08837h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Hyp含量。試劑盒檢測范圍為78 ng/mL-5000 ng/mL，適用于體外研究膠原代謝相關機制，如通過檢測細胞培養(yǎng)上清、實驗動物模型樣本或人體組織勻漿中的Hyp含量，探究器官纖維化進程、骨基質重塑動態(tài)或傷口愈合調控等科研課題，為組織工程、病理機制及藥物干預研究提供定量分析工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    N/A
  • 縮寫：
    Hyp
  • 種屬：
    Homo sapiens (Human)
  • 反應時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human Hyp in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %93
    Range %86-98
    1:2Average %103
    Range %97-107
    1:4Average %93
    Range %85-98
    1:8Average %97
    Range %91-101
  • 回收率：
    The recovery of human Hyp spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8680-94
    EDTA plasma (n=4)9692-105
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    50002.941 2.960 2.951 2.823
    25002.272 2.205 2.239 2.111
    12501.286 1.296 1.291 1.163
    6250.751 0.795 0.773 0.645
    312.50.388 0.400 0.394 0.266
    1560.322 0.342 0.332 0.204
    780.207 0.215 0.211 0.083
    00.124 0.132 0.128 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

平均分:
5.0分 - 1 個評價

樣品類型：細胞培養(yǎng)上清液

樣品信息：人

稀釋比：沒有稀釋

產品評價： 我用CSB-E08837h檢測脂多糖誘導人牙齦成纖維細胞炎癥模型中，標準曲線擬合度為0.9934，樣品的的OD值大約都在0.1以上，樣本換算濃度大概在78ng/mL左右

By 楊老師

相關問答

■ 常見問題解答
Q:

We are interested in sourcing Human, mouse, rat hydroxyproline,Hyp ELISA Kit from CusaBio. What would be our purchasing price for it?
Also, can you kindly clarify why they are a species specific elisa kit while hydroxyproline is a small molecule?

A:
Thanks for your inquiry.
I will ask our distributor in your area to give you a quote by email.
Hydroxyproline is small molecule and it is the same in different species. There are 3 reasons why we have species specific elisa kits.
1. For different species, the sample matrix is different and the characteristics of antibody used in the kits are also different.
2. The methods of these 3 kits are different. Human kit and mouse kit are based on double antibody sandwich method.
When the kit was developed, it was designed to mimic the presence of hydroxyproline in vivo. It can detect polymerized form. (including the polymerized form with other proteins and self-polymerization form). For human kit, capture antibody is mouse monoclonal antibody and detection antibody is goat polyclonal antibody; For mouse kit, capture antibody is rat monoclonal antibody and detection antibody is goat polyclonal antibody.
Rat kit is based on competitive method. The plate is coated with mouse monoclonal antibody. HRP conjugated HYP competes with the HYP in the sample. The kit can detect the endogenous total HYP, including free form and polymerized form.
3. The reason why different kits use different methods is based on a variety of factors. You can choose the appropriate kit to avoid matrix problems.

Pls let me know if you have any further questions. Thank you.