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  4. Human neuregulin-4 (NRG4) ELISA kit

Human neuregulin-4 (NRG4) ELISA kit

  • 中文名稱：
    人神經(jīng)調(diào)節(jié)蛋白4(NRG4)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL016080HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人神經(jīng)調(diào)節(jié)蛋白4(NRG4)酶聯(lián)免疫試劑盒（CSB-EL016080HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的NRG4含量。人神經(jīng)調(diào)節(jié)蛋白4（NRG4）是一種在神經(jīng)系統(tǒng)、心臟、乳腺等組織和器官中發(fā)揮作用的表皮生長因子，其通過與ErbB4受體結(jié)合，激活下游PI3K/Akt、STAT5等信號通路，促進細胞增殖、抑制細胞凋亡和改善細胞能量代謝。NRG4在肥胖、糖尿病、心血管疾病等多種疾病中發(fā)揮重要作用，成為潛在的治療靶點。試劑盒檢測范圍為125 pg/mL-8000 pg/mL，本產(chǎn)品適用于基礎(chǔ)科研中對NRG4在疾病模型、細胞實驗或動物組織中的表達水平分析，例如探究代謝相關(guān)信號通路機制或藥物干預(yù)效果評估，為代謝性疾病研究提供可靠的實驗工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    DKFZp779N0541 ELISA Kit; DKFZp779N1944 ELISA Kit; Heregulin 4 ELISA Kit; HRG4 ELISA Kit; Neuregulin 4 ELISA Kit; Neuregulin-4 ELISA Kit; NRG-4 ELISA Kit; NRG4 ELISA Kit; NRG4_HUMAN ELISA Kit; pro-neuregulin-4; membrane-bound isoform ELISA Kit; Pro-NRG4 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    125 pg/mL-8000 pg/mL
  • 靈敏度：
    31.25 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human NRG4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %90
    Range %86-95
    1:2Average %97
    Range %91-105
    1:4Average %95
    Range %90-101
    1:8Average %85
    Range %82-89
  • 回收率：
    The recovery of human NRG4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9087-96
    EDTA plasma (n=4)9894-103
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    80002.335 2.256 2.296 2.102
    40001.648 1.768 1.708 1.514
    20001.191 1.201 1.196 1.002
    10000.812 0.823 0.818 0.624
    5000.588 0.596 0.592 0.398
    2500.475 0.484 0.480 0.286
    1250.367 0.381 0.374 0.180
    00.193 0.195 0.194 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Low affinity ligand for the ERBB4 tyrosine kinase receptor. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. Does not bind to the ERBB1, ERBB2 and ERBB3 receptors.
  • 基因功能參考文獻：
    1. Nrg4 is highly and preferentially expressed in healthy adipocytes, while its expression was substantially reduced in obesity. Nrg4 activated endothelial angiogenic functions and angiogenesis both in vitro and in vivo. PMID: 29902456
    2. Serum NRG4 values were significantly elevated in the Gestational Diabetes Mellitus (GDM) group compared to the control group and is associated with metabolic parameters of GDM. PMID: 29282998
    3. circulating Nrg4 concentrations are inversely associated with subclinical atherosclerosis in obese adults; circulating Nrg4 might play a role in identifying patients at high risk for cardiovascular disease PMID: 27819316
    4. The circulating Nrg4 level is elevated in the prediabetic and diabetic patients compared to control and is an independent risk factor associated with diabetes. PMID: 27862843
    5. High serum Nrg4 levels are associated with type 2 diabetes mellitus. PMID: 27329015
    6. NRG4 and its receptor ErbB4 are present in human breast milk and developing human intestine, respectively. Thus, NRG4-ErbB4 signaling may be a novel pathway for therapeutic intervention or prevention in NEC. PMID: 25216938
    7. Nrg4 might have a protective role in the development of Non-alcoholic fatty liver disease (NAFLD). PMID: 26476959
    8. results establish Nrg4 as a brown fat-enriched endocrine factor with therapeutic potential for the treatment of obesity-associated disorders, including type 2 diabetes and nonalcoholic fatty liver disease (NAFLD). PMID: 25401691
    9. Pharmacological inhibition of PI3K/Akt signaling reversed the anti-apoptotic effects of NRG4, confirming the role of this cascade in NRG4-induced cell survival PMID: 23033483
    10. The recombinant neuregulin 4 induced the tyrosine phosphorylation of HER4. PMID: 21805036
    11. NRG4 overexpression is associated with advanced-stage prostate cancer PMID: 17545517
    12. describes a novel splice variant of the NRG4 gene, NRG4A2, which encodes a C-terminal region containing a predicted type I PDZ-binding peptide PMID: 17684490
    13. NRG4 was positive in almost all breast cancers studied. PMID: 17962208
    14. Neuregulin-4 expression is downregulated during urinary bladder cancer progression PMID: 15583696

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  • 亞細胞定位：
    [Pro-neuregulin-4, membrane-bound isoform]: Cell membrane; Single-pass type I membrane protein.; [Neuregulin-4]: Secreted.
  • 蛋白家族：
    Neuregulin family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 29862

    OMIM: 610894

    KEGG: hsa:145957

    STRING: 9606.ENSP00000378367

    UniGene: Hs.732438