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Human platelet-derived growth factor CC (PDGF-CC) ELISA Kit

  • 中文名稱:
    人血小板衍生生長因子CC(PDGF-CC)酶聯免疫試劑盒
  • 貨號:
    CSB-E13241h
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    人血小板衍生生長因子CC(PDGF-CC)酶聯免疫試劑盒(CSB-E13241h)為雙抗夾心法ELISA試劑盒,定量檢測血清、組織培養上清液、尿液、組織勻漿樣本中的PDGF-CC含量。PDGF-CC 即血小板衍生生長因子 CC,在細胞生長、增殖等生理過程中發揮作用。其異常表達與腫瘤、纖維化等疾病的發生發展相關。研究機制主要聚焦于其信號通路,阻斷該信號有望為相關疾病治療提供方向,目前是研究熱門靶點。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL,科研人員可通過該產品開展PDGF-CC在疾病模型中的表達水平監測、藥物干預效果評估或信號通路機制研究,為心血管生物學、腫瘤微環境及再生醫學等領域的體外實驗提供可靠數據支持。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    PDGF-CC
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, cell culture supernates, urine, tissue homogenates
  • 檢測范圍:
    0.156 ng/mL-10 ng/mL
  • 靈敏度:
    0.039 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cardiovascular
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human PDGF-CC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %92
    Range %88-96
    1:2Average %95
    Range %92-99
    1:4Average %94
    Range %89-101
    1:8Average %89
    Range %84-95
  • 回收率:
    The recovery of human PDGF-CC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9691-102
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.308 2.418 2.363 2.260
    51.887 2.032 1.960 1.857
    2.51.452 1.423 1.438 1.335
    1.250.782 0.821 0.802 0.699
    0.6250.477 0.497 0.487 0.384
    0.3120.325 0.346 0.336 0.233
    0.1560.215 0.205 0.210 0.107
    00.104 0.101 0.103  
     
  • 數據處理:
  • 貨期:
    3-5 working days

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