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  4. Mouse 8-Hydroxy-desoxyguanosine,8-OHdG ELISA Kit

Mouse 8-Hydroxy-desoxyguanosine,8-OHdG ELISA Kit

  • 中文名稱：
    小鼠8羥基脫氧鳥苷(8-OHdG)酶聯免疫試劑盒
  • 貨號：
    CSB-E10527m
  • 規格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    小鼠8羥基脫氧鳥苷(8-OHdG)酶聯免疫試劑盒（CSB-E10527m）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的8-OHdG含量。8-OHdG 是 DNA 氧化損傷的生物標志物。其背景在于機體受內外界因素影響產生氧化應激，導致 DNA 中鳥嘌呤氧化形成 8-OHdG。研究機制多圍繞檢測其含量來評估氧化應激水平、DNA 損傷程度，及與腫瘤、神經退行性疾病等的關聯。試劑盒檢測范圍為0.78 pg/mL-50 pg/mL，適用于神經退行性疾病模型、衰老機制研究、環境毒理學評估及代謝性疾病等科研領域，可為氧化損傷相關機制研究、藥物篩選及抗氧化劑功效評價提供數據支持，廣泛應用于基礎醫學、藥理學及生物化學等科研場景。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    8-OHdG
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.78 pg/mL-50 pg/mL
  • 靈敏度：
    0.195 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse 8-OHdG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 90  
    Range % 87-97  
    1:200 Average % 97  
    Range % 93-104  
    1:400 Average % 85  
    Range % 82-91  
    1:800 Average % 90  
    Range % 85-97  
  • 回收率：
    The recovery of mouse 8-OHdG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 89 85-93  
    EDTA plasma (n=4) 92 88-94  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    50 0.071 0.074 0.073    
    25 0.120 0.130 0.125    
    12.5 0.200 0.206 0.203    
    6.25 0.335 0.340 0.338    
    3.12 0.573 0.554 0.564    
    1.56 0.858 0.841 0.850    
    0.78 1.129 1.139 1.134    
    0 2.215 2.185 2.200    
  • 數據處理：
  • 貨期：
    3-5 working days

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