Mouse Activin B(ACV-B)ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠活化素B(ACV-B)酶聯(lián)免疫試劑盒（CSB-E15932m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ACV-B含量。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，適用于實(shí)驗(yàn)室研究，可廣泛用于評估動(dòng)物模型中ACV-B在卵巢功能、胚胎形成、代謝綜合征（如多囊卵巢或肥胖模型）中的表達(dá)變化，亦可用于探索其在組織再生或炎癥反應(yīng)中的調(diào)控機(jī)制。實(shí)驗(yàn)兼容多種生物樣本類型，為體外研究提供靈活選擇本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

ACV-B
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

6.25 pg/mL-400 pg/mL
靈敏度：

1.56 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ACV-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	92-105
1:2	Average %	94
	Range %	85-100
1:4	Average %	91
	Range %	86-98
1:8	Average %	96
	Range %	91-104

回收率：

The recovery of mouse ACV-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	90-95
EDTA plasma (n=4)	95	92-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.