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Mouse Carboxyterminal propeptide of type Ⅰ procollagen,PⅠCP ELISA Kit

  • 中文名稱:
    小鼠Ⅰ型前膠原羧基端肽(PⅠCP)酶聯免疫試劑盒
  • 貨號:
    CSB-E08080m
  • 規格:
    96T/48T
  • 價格:
    ¥3800/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    小鼠Ⅰ型前膠原羧基端肽(PⅠCP)酶聯免疫試劑盒(CSB-E08080m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿樣本中的PⅠCP含量。小鼠Ⅰ型前膠原羧基端肽(PⅠCP)是骨代謝的重要指標,其水平變化與骨吸收和骨形成密切相關。研究顯示,PⅠCP在多種疾病如骨折、骨質疏松和腫瘤骨轉移中扮演關鍵角色,其表達水平變化可作為疾病診斷和治療的潛在靶點。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL,適用于骨代謝相關疾病模型(如骨質疏松、骨發育異常)的實驗研究,也可用于肝纖維化、肺纖維化等組織修復與纖維化進程的機制探討,為體外研究Ⅰ型膠原代謝通路提供可靠的檢測工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Carboxyterminal propeptide of type 1 procollagen,P1CP,_x000D_ Carboxyterminal propeptide of type I procollagen,PICP
  • 縮寫:
    PⅠCP
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    31.25 pg/mL-2000 pg/mL
  • 靈敏度:
    7.81 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PⅠCP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 95  
    Range % 90-99  
    1:200 Average % 91  
    Range % 85-96  
    1:400 Average % 87  
    Range % 82-93  
    1:800 Average % 96  
    Range % 92-102  
  • 回收率:
    The recovery of mouse PⅠCP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 86-95  
    EDTA plasma (n=4) 92 88-97  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2000 2.821 2.731 2.776 2.630  
    1000 2.132 2.314 2.223 2.077  
    500 1.749 1.673 1.711 1.565  
    250 1.097 1.150 1.124 0.978  
    125 0.695 0.646 0.671 0.525  
    62.5 0.407 0.387 0.397 0.251  
    31.25 0.332 0.317 0.325 0.179  
    0 0.148 0.144 0.146    
  • 數據處理:
  • 貨期:
    3-5 working days

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