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中文名稱:小鼠透明質酸(HA)酶聯免疫試劑盒
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貨號:CSB-E08121m
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:62.5 pg/mL-4000 pg/mL
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靈敏度:15.6 pg/mL
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規格:96T/48T
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價格:¥3800/¥2500
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促銷:
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其他:
產品詳情
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產品描述:小鼠透明質酸 (Hyaluronic Acid, HA) 酶聯免疫試劑盒采用雙抗體夾心法 ELISA 技術,專門用于定量檢測小鼠血清、血漿及組織勻漿樣本中的透明質酸 (HA) 含量。
1、生物學意義與應用領域:透明質酸 (HA) 是一種天然存在的非硫酸化糖胺聚糖,是細胞外基質和滑液的主要成分,具有卓越的保水性和生物相容性。關節炎研究: 監測關節滑液及血清中 HA 水平,評估軟骨降解程度、滑膜炎癥狀態及骨關節炎進展。皮膚再生與修復: 探究 HA 在傷口愈合、皮膚保濕機制及抗衰老研究中的動態變化。腫瘤微環境: 分析腫瘤組織中 HA 的異常積聚對癌細胞侵襲、轉移及藥物耐藥性的影響。代謝與纖維化疾病: 探索 HA 代謝紊亂在肝纖維化、肥胖及相關代謝綜合征中的作用機制。本試劑盒適用于科研領域中對不同病理狀態下 HA 表達水平的動態監測,以及評估藥物或干預措施對 HA 代謝通路的調節效果。
2、技術參數:
檢測方法: 雙抗體夾心法 ELISA;
檢測范圍: 62.5 pg/mL - 4000 pg/mL;
適用樣本: 血清、血漿、組織勻漿;
適用物種: 小鼠 (Mouse)。3、注意事項:
本品僅限科學研究使用,不可用于臨床診斷。實驗具體操作步驟、試劑配制及數據分析方法,請嚴格參照隨附的產品說明書執行。 -
別名:N/A
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縮寫:HA
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種屬:Mus musculus (Mouse)
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反應時間:1-5h
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上樣量:50-100ul
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檢測波長:450 nm
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研究領域:Cell Biology
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse HA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 89 Range % 82-99 1:200 Average % 93 Range % 89-101 1:400 Average % 102 Range % 98-106 1:800 Average % 97 Range % 92-102 -
回收率:
The recovery of mouse HA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 90 87-94 EDTA plasma (n=4) 97 93-107 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 4000 2.755 2.856 2.806 2.652 2000 2.027 2.128 2.078 1.924 1000 1.435 1.455 1.445 1.291 500 0.850 0.861 0.856 0.702 250 0.526 0.515 0.521 0.367 125 0.358 0.348 0.353 0.199 62.5 0.267 0.261 0.264 0.110 0 0.154 0.153 0.154 -
數據處理:
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貨期:3-5 working days
引用文獻
- Targeting hyaluronan synthesis enhances the therapeutic effectiveness of biologics in inflammatory bowel disease P Xiao, Z Chen, X Cai, W Xia, X Liu, Z Song,JCI insight,2025
- Идиопатический легочный фиброз (ИЛФ) АА Ярославцева,/,2024
- Dietary supplementation with α-ionone alleviates chronic UVB exposure-induced skin photoaging in mice R Geng,Food & function,2024
- Dietary Isoeugenol Supplementation Attenuates Chronic UVB-Induced Skin Photoaging and Modulates Gut Microbiota in Mice Y Chen,Nutrients,2024
- Revitalizing Photoaging Skin through Eugenol in UVB-Exposed Hairless Mice: Mechanistic Insights from Integrated Multi-Omics F Feng,Antioxidants,2024
- Farnesoid X receptor activation is required for the anti-inflammatory and anti-oxidative stress effects of Alisol B 23-acetate in carbon tetrachloride-induced liver fibrosis in mice L Zhang,International immunopharmacology,2023
- Anti-inflammatory and Antifibrotic Potential of Longidaza in the Bleomycin-induced Pulmonary Fibrosis A Pakhomova,/,2023
- Combination leflunomide and methotrexate impedes the recovery of liver fibrosis, partly through inhibition of myeloid cell admittance Mengxin Lin, et al,Molecular Medicine Reports,2019
- A Polysaccharide from Amusium Pleuronectes Combined with Praziquantel Treatment Ameliorates Hepatic Fibrosis in Schistosoma Japonicum-Infected Mice Tang X.et al,Med Sci Monit,2018
- Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica. Xie Y.et al,Cell Mol Immunol.,2016
- Role of Hematopoietic Stem Cells in Inflammation of the Pancreas during Diabetes Mellitus. Dygai AM.et al,Bull Exp Biol Med.,2016
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