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Mouse Progesterone,PROG ELISA Kit

  • 中文名稱:
    小鼠孕激素/孕酮(PROG)酶聯免疫試劑盒
  • 貨號:
    CSB-E05104m
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    0.3 ng/mL-10 ng/mL
  • 靈敏度:
    0.2 ng/mL
  • 規格:
    96T/48T
  • 價格:
    ¥3800/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    小鼠孕激素/孕酮(PROG)酶聯免疫試劑盒(CSB-E05104m)為競爭法ELISA試劑盒,定量檢測血清、血漿樣本中的PROG含量。小鼠孕激素/孕酮(PROG)在妊娠和生殖過程中發揮關鍵作用,調控子宮內膜和胎盤發育,維持妊娠穩定。其作用機制涉及調節基因表達、影響細胞增殖和凋亡,并與孕酮受體結合,參與激素信號傳導。試劑盒檢測范圍為0.3 ng/mL-10 ng/mL,靈敏度為0.2 ng/mL。適用于生殖生物學、內分泌調控機制研究及藥物/化合物對生殖系統影響的評估,例如探究妊娠動物模型激素動態、卵巢功能異常研究或激素替代治療相關實驗。支持科研人員高效開展小鼠生殖健康、代謝調控等領域的基礎研究。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    PROG
  • 種屬:
    Mus musculus (Mouse)
  • 反應時間:
    1-5h
  • 上樣量:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<15%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<15%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PROG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    96

    Range %

    92-104

    1:2

    Average %

    93

    Range %

    91-97

    1:4

    Average %

    102

    Range %

    98-106

    1:8

    Average %

    87

    Range %

    82-94

  • 回收率:

    The recovery of mouse PROG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    94

    90-97

    EDTA plasma (n=4)

    89

    85-94

  • 標準曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    10

    0.175

    0.189

    0.182

    5

    0.452

    0.456

    0.454

    2.5

    0.759

    0.783

    0.771

    0.86

    1.175

    1.251

    1.213

    0.3

    1.647

    1.685

    1.666

    0

    2.085

    2.130

    2.107

  • 數據處理:
  • 貨期:
    3-5 working days

引用文獻

產品評價

平均分:
5.0分 - 1 個評價

樣品類型:血清

樣品信息:小鼠

稀釋比:沒有稀釋

產品評價: 我用CSB-E05104m檢測小鼠血清, 組 od od-空白 1+(x/C)^B 濃度 ave 對照 C36 0.82 0.7843 24.54654803 0.47730038 1.187548202 C29 0.4774 0.4417 6.960100594 1.783379399 C32 0.6501 0.6144 14.1283102 0.858463521 C34 0.5804 0.5447 10.84184426 1.130691667 C35 0.5093 0.4736 8.042388335 1.538055725 C30 0.5472 0.5115 9.466873123 1.300665137 C31 0.5956 0.5599 11.51183861 1.06258015 C68 0.6491 0.6134 14.07723803 0.861721692 C69 0.5457 0.51 9.407580558 1.309112341 C27 0.5071 0.4714 7.964361562 1.553512003 模型 M93 0.623 0.5873 12.7850009 0.9528223 0.928424824 M86 0.5064 0.4707 7.939640929 1.55847227 M5 0.632 0.5963 13.22176892 0.920038614 M9 0.6512 0.6155 14.18462346 0.854898189 M3 0.8003 0.7646 23.15598224 0.508341343 M32 0.6163 0.5806 12.46584383 0.978230816 M45 0.623 0.5873 12.7850009 0.9528223 M43 0.6613 0.6256 14.70825487 0.823052987 M91 0.7442 0.7085 19.46291702 0.612312557 M51 0.582 0.5463 10.91116227 1.123256869 低 D88 0.7105 0.6748 17.430688 0.688318485 0.950553217 D83 0.5398 0.5041 9.176712347 1.343043317 D97 0.7678 0.7321 20.96880975 0.565494908 D84 0.8916 0.8559 30.02115395 0.383039317 D82 0.8069 0.7712 23.61637202 0.497659491 D89 0.5019 0.4662 7.781942693 1.590856623 D16 0.7261 0.6904 18.35428318 0.651689323 D14 0.5545 0.5188 9.758899877 1.260558846 D15 0.6345 0.5988 13.34473782 0.91119575 D47 0.4988 0.4631 7.674531748 1.613676111 中 Z94 0.513 0.4773 8.174757873 1.512509579 0.933564465 Z81 0.6411 0.6054 13.6728247 0.888380888 Z91 0.4853 0.4496 7.218362913 1.7181559 Z2 0.5231 0.4874 8.543418904 1.445533779 Z8 0.8155 0.7798 24.22456047 0.484170907 Z42 0.801 0.7653 23.20455029 0.50719448 Z39 1.3105 1.2748 76.28816628 0.126718325 Z34 0.5609 0.5252 10.01967723 1.226720233 Z41 0.6683 0.6326 15.07815003 0.8018905 Z93 0.7385 0.7028 19.10947609 0.624370061 高 G98 0.5173 0.4816 8.330398474 1.483510814 0.921326038 G92 0.6466 0.6109 13.95006425 0.869938463 G100 0.5232 0.4875 8.547122843 1.444890188 G95 0.9204 0.8847 32.41285428 0.351853338 G96 0.5515 0.5158 9.638191123 1.276842042 G7 0.5482 0.5125 9.506536307 1.295073281 G4 0.7001 0.6644 16.83123384 0.714243724 G48 0.9476 0.9119 34.77353223 0.325278665 G46 0.9154 0.8797 31.98970949 0.357031298 G52 0.5883 0.5526 11.18685944 1.094598572

By 楊老師

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