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Mouse calcitonin gene related peptide (CGRP) ELISA kit

  • 中文名稱:
    小鼠降鈣素基因相關肽(CGRP)酶聯免疫試劑盒
  • 貨號:
    CSB-EQ027706MO
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    小鼠降鈣素基因相關肽(CGRP)酶聯免疫試劑盒(CSB-EQ027706MO)檢測血清、血漿、組織勻漿樣本中的CGRP含量。CGRP(降鈣素基因相關肽)是一種神經肽,主要在感覺神經纖維表達,如C和Aδ感覺纖維,廣泛分布于全身,尤其在血管周圍。CGRP在偏頭痛的發病機制中起關鍵作用,通過促進神經源性炎癥和顱腦血管擴張引發偏頭痛。近年來,針對CGRP及其受體的藥物研發成為偏頭痛治療的熱點。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL,支持科研人員探究CGRP在神經科學、藥理學或疾病模型中的動態變化,例如分析疼痛模型中神經遞質釋放規律、評估藥物對CGRP通路的調控作用,或研究心血管疾病與神經肽關聯機制。本試劑盒適用于多種生物樣本類型,為神經生物學、免疫學及代謝性疾病等基礎研究領域提供標準化檢測工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Beta type CGRP、Calcitonin gene-related peptide II、CGRPII、Calcitonin gene-related peptide 2、CALCB
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    15.6 pg/mL-1000 pg/mL
  • 靈敏度:
    3.9 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 86-98  
    1:2 Average % 92  
    Range % 88-97  
    1:4 Average % 90  
    Range % 85-96  
    1:8 Average % 94  
    Range % 88-99  
  • 回收率:
    The recovery of mouse CGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 89-97  
    EDTA plasma (n=4) 96 91-99  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.342 2.321 2.332 2.178  
    500 1.772 1.760 1.766 1.612  
    250 1.250 1.241 1.246 1.092  
    125 0.799 0.789 0.794 0.640  
    62.5 0.499 0.487 0.493 0.339  
    31.25 0.364 0.341 0.353 0.199  
    15.6 0.253 0.244 0.249 0.095  
    0 0.156 0.152 0.154    
  • 數據處理:
  • 貨期:
    3-5 working days

引用文獻

產品評價

平均分:
5.0分 - 2 個評價

樣品類型:組織勻漿液 小鼠神經節組織

樣品信息:小鼠

稀釋比:沒有稀釋

產品評價: ELISA試劑盒在檢測CGRP時展現了較高的靈敏度和特異性。在小鼠drg樣本中,重復性良好。試劑盒的操作簡便,且廠家提供了詳細的實驗步驟和在線技術支持,幫助解決了實驗中的問題。ELISA試劑盒的檢測范圍(最低和最高濃度)也適應實驗的需求。具備高靈敏度,能夠在低濃度下準確檢測CGRP。

By 閆老師

樣品類型:組織勻漿液 小鼠肺組織勻漿液

樣品信息:小鼠

稀釋比:1:50

產品評價: 我使用CSB-EQ027706MO檢測小鼠肺組織勻漿液樣本,標準曲線的 OD 值在 0.176 - 1.749 之間呈現良好線性關系,樣本 OD 值大致在 0.2 - 1.7 區間。試劑盒的重復性較好,多次平行實驗結果波動比較小。從操作體驗來說,試劑預分裝設計十分貼心,減少了繁瑣的準備步驟,節省了時間。而且靈敏度較高,能精準檢測到CGRP,為科研數據提供了可靠支撐,是一款非常值得信賴的 ELISA 試劑盒。

By 王老師

靶點詳情

  • 功能:
    CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role.
  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    Calcitonin family
  • 組織特異性:
    Detected in nerve cells of cerebrum, hippocampus and pons/midbrain in newborns, and only in nerve cells of pons/midbrain in adult.
  • 數據庫鏈接: