Rabbit Fibronectin(FN1) ELISA kit

產(chǎn)品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔纖連蛋白(FN1)酶聯(lián)免疫試劑盒（CSB-EL008759RB）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FN1含量。FN1即纖連蛋白 1，是細胞外基質(zhì)的重要成分。它參與細胞黏附、遷移、增殖等多種生理過程。在研究機制方面，F(xiàn)N1 可與多種受體相互作用，調(diào)節(jié)細胞信號通路。其異常表達與腫瘤、纖維化等疾病密切相關(guān)，是潛在治療靶點。試劑盒檢測范圍為1.25 ng/mL-20 ng/mL，適用于實驗室對FN1蛋白表達水平的動態(tài)監(jiān)測，可廣泛用于心血管疾病模型、纖維化機制研究、腫瘤微環(huán)境分析等基礎(chǔ)科研領(lǐng)域，為探索細胞外基質(zhì)調(diào)控機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

FN1Fibronectin ELISA Kit; FN ELISA Kit; Fragment ELISA Kit
縮寫：

FN1
Uniprot No.：

Q28749
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.25 ng/mL-20 ng/mL
靈敏度：

1.25 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit FN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	95
	Range %	92-98
1:200	Average %	103
	Range %	98-108
1:400	Average %	98
	Range %	94-102
1:800	Average %	92
	Range %	87-97

回收率：

The recovery of rabbit FN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	96-101
EDTA plasma (n=4)	89	82-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
亞細胞定位：

Secreted, extracellular space, extracellular matrix.
組織特異性：

Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix.
數(shù)據(jù)庫鏈接：

STRING: 9986.ENSOCUP00000008718

UniGene: Ocu.1875