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  4. Rabbit Interleukin 4,IL-4 ELISA Kit

Rabbit Interleukin 4,IL-4 ELISA Kit

  • 中文名稱：
    兔子白介素4(IL-4)酶聯免疫試劑盒
  • 貨號：
    CSB-E06902Rb
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.625 pg/mL-40 pg/mL
  • 靈敏度：
    0.156 pg/mL
  • 規格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    兔子白介素4(IL-4)酶聯免疫試劑盒（CSB-E06902Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL4含量。IL4是一種細胞因子，在免疫系統中扮演重要角色。它主要由Th2細胞等分泌，能促進B細胞增殖分化、調節免疫球蛋白類別轉換。在研究機制上，其通過與IL4受體結合激活下游信號通路，參與過敏、哮喘等疾病發生發展，是相關疾病治療的潛在靶點。試劑盒檢測范圍為0.625 pg/mL-40 pg/mL，適用于動物模型中的Th2型免疫應答研究、炎癥性疾病機制探索或感染過程中細胞因子動態監測本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 反應時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %80-92
    1:2Average %100
    Range %91-105
    1:4Average %97
    Range %92-105
    1:8Average %94
    Range %86-98
  • 回收率：
    The recovery of rabbit IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9389-98
    EDTA plasma (n=4)9590-100
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    402.804 2.786 2.795 2.706
    202.280 2.176 2.228 2.139
    101.544 1.443 1.494 1.405
    50.899 0.876 0.888 0.799
    2.50.443 0.423 0.433 0.344
    1.250.230 0.226 0.228 0.139
    0.6250.154 0.149 0.152 0.063
    00.090 0.088 0.089 　
  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

平均分:
5.0分 - 1 個評價

樣品類型：組織勻漿液 兔肺組織勻漿取上清

樣品信息：其他 兔子

稀釋比：其他 1：5

產品評價： 我用CSB.E06902Rb檢測正常組和實驗組的兔肺組織勻漿上清液，標準曲線的OD值為0.063-2.038。標準曲線有很好的線性范圍，測量結果準確。

By 馬老師

靶點詳情

  • 功能：
    Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    IL-4/IL-13 family
  • 數據庫鏈接：