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  4. Rabbit Vascular Endothelial cell Growth Factor,VEGF ELISA Kit

Rabbit Vascular Endothelial cell Growth Factor,VEGF ELISA Kit

  • 中文名稱：
    兔血管內皮細胞生長因子(VEGF)酶聯免疫試劑盒
  • 貨號：
    CSB-E08524Rb
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1.56 pg/mL-100 pg/mL
  • 靈敏度：
    0.39 pg/mL
  • 規格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    兔血管內皮細胞生長因子(VEGF)酶聯免疫試劑盒（CSB-E08524Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的VEGF含量。VEGF即血管內皮生長因子，在腫瘤血管生成、傷口愈合等生理過程中起關鍵作用。其背景在于它能促進內皮細胞增殖、遷移和血管生成。研究機制方面，主要是與受體結合，激活下游信號通路，誘導血管生成，是腫瘤治療等領域的重要靶點。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL，適用于科研場景下探究血管生成相關機制、評估疾病模型中VEGF的動態變化，或用于藥物研發中靶向分子效應的體外檢測。可為心血管疾病、腫瘤微環境等領域的基礎研究提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    VEGF
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 反應時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %85
    Range %81-95
    1:2Average %100
    Range %91-106
    1:4Average %94
    Range %92-105
    1:8Average %90
    Range %86-98
  • 回收率：
    The recovery of rabbit VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-98
    EDTA plasma (n=4)9791-100
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.538 2.540 2.539 2.403
    502.012 2.022 2.017 1.881
    251.137 1.130 1.134 0.998
    12.50.602 0.651 0.627 0.491
    6.250.375 0.378 0.377 0.241
    3.120.279 0.285 0.282 0.146
    1.560.168 0.176 0.172 0.036
    00.131 0.141 0.136 　
  • 數據處理：
  • 貨期：
    3-5 working days

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相關問答

■ 常見問題解答
Q:

Please provide the following information regarding Rabbit Vascular Endothelial cell Growth Factor, VEGF ELISA Kit (CSB-E08524Rb)
a. Can this kit detect human VEGF?

A:
Thanks for your inquiry.
About Vascular Endothelial cell Growth Factor，there is VEGFA～D for human while there is only VEGF for rabbit.
CSB-E08524Rb This kit is designed according to: https://www.uniprot.org/uniprot/Q866G4.
It detects VEGF and it can not test human samples.
Pls let me know if you have any further questions. Thank you.