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Rabbit complement fragment 5a,C5a ELISA Kit

  • 中文名稱:
    兔子補體片斷5a(C5a)酶聯免疫試劑盒
  • 貨號:
    CSB-E13247Rb
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    兔子補體片斷5a(C5a)酶聯免疫試劑盒(CSB-E13247Rb)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的C5a含量。C5a是補體系統激活過程中產生的重要炎癥介質。其背景在于補體系統是機體免疫防御的關鍵部分。研究機制方面,C5a可與受體結合,引發炎癥細胞趨化、活化,放大炎癥反應,在感染、自身免疫病等多種疾病病理進程中發揮重要作用。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL,科研人員可將其應用于炎癥信號通路研究、免疫調控機制探索、疾病動物模型評估(如感染性疾病或自身免疫模型),以及抗炎藥物研發過程中生物標志物的動態監測。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    C5a
  • 種屬:
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.625 ng/mL-40 ng/mL
  • 靈敏度:
    0.156 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %86
    Range %82-90
    1:2Average %92
    Range %88-96
    1:4Average %94
    Range %90-98
    1:8Average %102
    Range %98-106
  • 回收率:
    The recovery of rabbit C5a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8783-93
    EDTA plasma (n=4)10197-105
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    402.821 2.988 2.905 2.813
    202.564 2.414 2.489 2.397
    101.767 1.746 1.757 1.665
    50.991 0.974 0.983 0.891
    2.50.484 0.496 0.490 0.398
    1.250.286 0.297 0.292 0.200
    0.6250.157 0.162 0.160 0.068
    00.091 0.093 0.092
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價