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  4. Rabbit interleukin 12 (IL-12/P70) ELISA kit

Rabbit interleukin 12 (IL-12/P70) ELISA kit

  • 中文名稱：
    兔白介素12(IL-12/P70)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EQ027628RB
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    兔白介素12(IL-12/P70)酶聯(lián)免疫試劑盒（CSB-EQ027628RB）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL12/P70含量。IL12/P70是重要的免疫靶點(diǎn)。它由p35和p40亞基組成，在調(diào)節(jié)免疫細(xì)胞功能、促進(jìn)Th1細(xì)胞分化等方面發(fā)揮關(guān)鍵作用。其研究機(jī)制主要聚焦于調(diào)控免疫應(yīng)答相關(guān)信號通路。深入研究它有望為免疫相關(guān)疾病治療提供新策略。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，該產(chǎn)品適用于實(shí)驗(yàn)動(dòng)物模型的免疫調(diào)控研究、感染性疾病機(jī)制探索、疫苗效力評估及炎癥相關(guān)科研項(xiàng)目，為研究IL-12/P70在免疫微環(huán)境中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IL12/P70, Interleukin-12/P70
  • 縮寫：
    IL-12/P70
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    3.12 pg/mL-200 pg/mL
  • 靈敏度：
    0.78 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %93
    Range %89-95
    1:2Average %90
    Range %83-99
    1:4Average %95
    Range %92-102
    1:8Average %93
    Range %86-99
  • 回收率：
    The recovery of rabbit IL-12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9586-110
    EDTA plasma (n=4)9789-99
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.096 2.108 2.102 1.938
    1001.678 1.651 1.665 1.501
    501.254 1.237 1.246 1.082
    250.896 0.905 0.901 0.737
    12.50.614 0.642 0.628 0.464
    6.250.378 0.364 0.371 0.207
    3.120.252 0.249 0.251 0.087
    00.161 0.166 0.164
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)