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  4. Rat Dihydrotestosterone,DHT ELISA Kit

Rat Dihydrotestosterone,DHT ELISA Kit

  • 中文名稱：
    大鼠雙氫睪酮(DHT)酶聯免疫試劑盒
  • 貨號：
    CSB-E07879r
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    10 pg/mL-2000 pg/mL
  • 靈敏度：
    5 pg/mL
  • 規格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    大鼠雙氫睪酮(DHT)酶聯免疫試劑盒（CSB-E07879r）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的DHT含量。DHT（分布式哈希表）是一種去中心化網絡技術，通過將資源信息分散存儲在多個節點上，實現高效組織和定位。每個節點負責存儲部分資源信息和成員聯系信息，通過哈希算法和節點ID關聯，實現資源查找和節點通信。試劑盒檢測范圍為10 pg/mL-2000 pg/mL，科研人員可將其應用于內分泌調控研究、雄性生殖生物學實驗、藥物開發中激素代謝評估，或探究前列腺疾病、脫發等病理模型的激素水平變化本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    N/A
  • 縮寫：
    DHT
  • 種屬：
    Rattus norvegicus (Rat)
  • 反應時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat DHT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 102  
    Range % 98-106  
    1:2 Average % 93  
    Range % 89-97  
    1:4 Average % 95  
    Range % 89-99  
    1:8 Average % 102  
    Range % 98-106  
  • 回收率：
    The recovery of rat DHT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-95  
    EDTA plasma (n=4) 102 97-106  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    2000 0.156 0.160 0.158 　  
    500 0.312 0.329 0.321 　  
    160 0.498 0.528 0.513 　  
    40 0.818 0.787 0.803    
    10 1.316 1.278 1.297    
    0 1.960 1.954 1.957    
  • 本試劑盒所含材料：
    • A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-rabbit DHT antibody.
    • Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
    • Anti-mouse DHT Antibody 1 x 6 ml -- Act as the detection antibody.
    • HRP-conjugate 1 x 6 ml -- Bind to the DHT antibody, and HRP catalyzes the TMB to elicit a chromogenic reaction.
    • Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
    • Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
    • Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color.
    • Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells)
    • An Instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數據處理：
  • 貨期：
    3-5 working days

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