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  4. Rat Free Testosterone,F-TESTO ELISA Kit

Rat Free Testosterone,F-TESTO ELISA Kit

  • 中文名稱：
    大鼠游離睪酮(F-TESTO)酶聯免疫試劑盒
  • 貨號：
    CSB-E05097r
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    0.3 pg/mL-60 pg/mL
  • 靈敏度：
    0.15 pg/mL
  • 規格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    大鼠游離睪酮(F-TESTO)酶聯免疫試劑盒（CSB-E05097r）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的F-TESTO含量。試劑盒檢測范圍為0.3 pg/mL-60 pg/mL，該產品可為生殖生物學研究、環境內分泌干擾物評估、睪丸功能障礙模型驗證以及雄激素相關藥物作用機制探索提供可靠數據支持，廣泛應用于神經內分泌調控、性腺衰老、代謝綜合征等動物實驗的激素水平檢測，為科研工作者提供標準化的睪酮活性狀態分析工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    F-TESTO
  • 種屬：
    Rattus norvegicus (Rat)
  • 反應時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Epigenetics and Nuclear Signaling
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat F-TESTO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 90-101  
    1:2 Average % 95  
    Range % 89-99  
    1:4 Average % 94  
    Range % 90-98  
    1:8 Average % 95  
    Range % 90-100  
  • 回收率：
    The recovery of rat F-TESTO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 89-104  
    EDTA plasma (n=4) 94 88-99  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    60 0.202 0.205 0.204    
    15 0.359 0.342 0.351    
    4.8 0.610 0.623 0.617    
    1.2 1.007 1.012 1.010    
    0.3 1.464 1.457 1.461    
    0 2.439 2.454 2.447    
  • 數據處理：
  • 貨期：
    3-5 working days

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