Rat rudimental bovine serum albumin(BSA) check-up ELISA kit

文獻引用 (2) 說明書下載

產品詳情

產品描述：

大鼠的牛血清白蛋白殘留檢測酶聯免疫試劑盒（CSB-E08634r）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的BSA含量。靶點研究旨在發現疾病過程中的特定分子或細胞結構，通過藥物與靶點相互作用產生治療效果。BSA（牛血清白蛋白）作為一種常用實驗材料，在免疫實驗中常用作封閉劑，其研究背景涉及靶點的發現、篩選和驗證，研究機制則包括基因組學、蛋白質組學和生物信息學等多學科技術。試劑盒檢測范圍為0.312 μg/mL-80 μg/mL，靈敏度為0.312 μg/mL。適用于生物制品生產流程質控、實驗動物血清樣本中異源蛋白殘留分析，以及生物醫藥研究中BSA清除工藝的優化驗證本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

BSA
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 μg/mL-80 μg/mL
靈敏度：

0.312 μg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat BSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	90-110
1:2	Average %	95
	Range %	89-106
1:4	Average %	95
	Range %	92-100
1:8	Average %	96
	Range %	92-99

回收率：

The recovery of rat BSA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-102
EDTA plasma (n=4)	96	89-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.