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  4. Sheep Interleukin 4(IL-4)ELISA Kit

Sheep Interleukin 4(IL-4)ELISA Kit

  • 中文名稱：
    綿羊白介素4(IL-4)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E15963Sh
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    綿羊白介素4(IL-4)酶聯(lián)免疫試劑盒（CSB-E15963Sh）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL4含量。IL4是一種由活化的T淋巴細(xì)胞、肥大細(xì)胞、嗜堿性粒細(xì)胞產(chǎn)生的多效性細(xì)胞因子，在免疫調(diào)節(jié)中起關(guān)鍵作用。它通過誘導(dǎo)B細(xì)胞分化、促進抗體產(chǎn)生和調(diào)節(jié)Th2細(xì)胞介導(dǎo)的免疫反應(yīng)，在過敏性疾病和腫瘤免疫中扮演重要角色。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，適用于免疫學(xué)研究、炎癥性疾病模型分析、疫苗免疫效果評估等科研領(lǐng)域，可用于探究綿羊感染性疾病、寄生蟲免疫應(yīng)答及自身免疫機制，為其提供精準(zhǔn)的蛋白定量數(shù)據(jù)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IL4 ELISA Kit; IL-4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Ovis aries (Sheep)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    1.56 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %86-95
    1:2Average %102
    Range %97-105
    1:4Average %95
    Range %90-97
    1:8Average %97
    Range %93-100
  • 回收率：
    The recovery of sheep IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-97
    EDTA plasma (n=4)9690-99
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.084 1.958 2.021 1.869
    2001.300 1.276 1.288 1.136
    1000.828 0.817 0.823 0.671
    500.518 0.529 0.524 0.372
    250.333 0.343 0.338 0.186
    12.50.281 0.290 0.286 0.134
    6.250.231 0.224 0.228 0.076
    00.154 0.150 0.152 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
  • 基因功能參考文獻：
    1. Local production of the prototypic immunomodulatory cytokine IL-4 in transplanted corneal endothelium induces eosinophilia, inflammation, and rejection. PMID: 16081789
    2. Early IL-4 gene expression in abomasum is associated with resistance to Haemonchus infection. PMID: 27059919
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    IL-4/IL-13 family
  • 數(shù)據(jù)庫鏈接：

    KEGG: oas:443327

    UniGene: Oar.377



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