SMAD5單克隆抗體（CUSABIO貨號(hào)：CSB-MA052999）是針對(duì)TGF-β信號(hào)通路關(guān)鍵調(diào)控因子SMAD5開發(fā)的科研專用試劑，該靶點(diǎn)通過介導(dǎo)骨形態(tài)發(fā)生蛋白（BMP）信號(hào)傳導(dǎo)參與細(xì)胞增殖、分化及胚胎發(fā)育調(diào)控。本產(chǎn)品采用高特異性雜交瘤技術(shù)制備，經(jīng)ELISA、Western Blot（WB）、免疫細(xì)胞化學(xué)（ICC）及流式細(xì)胞術(shù)（FC）多平臺(tái)驗(yàn)證，可精準(zhǔn)識(shí)別人類、豬、大鼠及小鼠等哺乳動(dòng)物來源的SMAD5蛋白，適用于腫瘤微環(huán)境研究、干細(xì)胞定向分化實(shí)驗(yàn)及骨骼發(fā)育相關(guān)機(jī)制探索。其優(yōu)異的種屬交叉反應(yīng)性為跨物種比較研究提供便利，特別適合開展BMP/SMAD信號(hào)通路在癌癥轉(zhuǎn)移、組織再生修復(fù)中的功能解析，以及基因敲除模型驗(yàn)證等實(shí)驗(yàn)場景。該抗體在4℃長期儲(chǔ)存條件下保持穩(wěn)定活性，重復(fù)實(shí)驗(yàn)結(jié)果一致性高，是探索TGF-β超家族信號(hào)網(wǎng)絡(luò)調(diào)控機(jī)制的高效工具。通過優(yōu)化抗原表位篩選工藝，有效降低非特異性結(jié)合干擾，助力研究人員在蛋白質(zhì)相互作用、亞細(xì)胞定位分析等前沿領(lǐng)域獲得可靠數(shù)據(jù)。

mothers against decapentaplegic homolog 5 SMA- and MAD-related protein 5 SMAD, mothers against DPP homolog 5 MAD, mothers against decapentaplegic homolog 5 mothers against decapentaplegic, drosophila, homolog of, 5

Mouse

Human,Pig,Rat,Mouse

Recombinant fragment, corresponding to amino acids 239aa-372aa of Human SMAD5 (C-terminus).

Homo sapiens (Human)

Monoclonal

IgG1

Antigen Affinity Purified

14E2

It differs from different batches. Please contact us to confirm it.

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol

ELISA,WB,ICC,FC

Application	Recommended Dilution
WB	1:500-1:5000
ICC	1:50-1:200

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.