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Recombinant Human Asialoglycoprotein receptor 1 (ASGR1)-Detergent (Active)

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  • 品名簡稱：
    Recombinant Human ASGR1 protein-Detergent (Active)
  • 貨號：
    CSB-MP002207HU(A4)-D
  • 說明書：
  • 規(guī)格：
    ￥7350
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized Human ASGR1 at 2 μg/ml on an Nickel Coated plate can bind Anti-ASGR1 recombinant antibody (CSB-RA002207MA3HU). The EC50 is 2.715-3.605 ng/mL. Biological Activity Assay
    • Activity
      ASGR1 Recombinant Monoclonal Antibody (CSB-RA002207MA3HU) captured on Protein A Chip can bind Recombinant Human ASGR1 with an affinity constant of 0.716 nM as detected by MetaSPR Assay (WeSPRTM 200). Biological Activity Assay
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 85% as determined by SDS-PAGE.
  • 內(nèi)毒素：
    Less than 1.0 EU/ug as determined by LAL method.
  • 生物活性：
    ①M(fèi)easured by its binding ability in a functional ELISA. Immobilized Human ASGR1 at 2 μg/mL on an Nickel Coated plate can bind Anti-ASGR1 recombinant antibody (CSB-RA002207MA3HU). The EC50 is 2.715-3.605 ng/mL.
    ②ASGR1 Recombinant Monoclonal Antibody (CSB-RA002207MA3HU) captured on Protein A Chip can bind Recombinant Human ASGR1 with an affinity constant of 0.716 nM as detected by MetaSPR Assay (WeSPRTM 200).
  • 基因名：
  • Uniprot No.：
  • 別名：
    Asialoglycoprotein receptor 1; ASGP-R 1; ASGPR 1; C-type lectin domain family 4 member H1; Hepatic lectin H1; HL-1
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full Length
  • 來源：
    Mammalian cell
  • 分子量：
    34.6 kDa
  • 表達(dá)區(qū)域：
    1-291aa
  • 氨基酸序列
    MTKEYQDLQHLDNEESDHHQLRKGPPPPQPLLQRLCSGPRLLLLSLGLSLLLLVVVCVIGSQNSQLQEELRGLRETFSNFTASTEAQVKGLSTQGGNVGRKMKSLESQLEKQQKDLSEDHSSLLLHVKQFVSDLRSLSCQMAALQGNGSERTCCPVNWVEHERSCYWFSRSGKAWADADNYCRLEDAHLVVVTSWEEQKFVQHHIGPVNTWMGLHDQNGPWKWVDGTDYETGFKNWRPEQPDDWYGHGLGGGEDCAHFTDDGRWNDDVCQRPYRWVCETELDKASQEPPLL
  • 蛋白標(biāo)簽：
    C-terminal 10xHis-tagged
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Lyophilized from a 0.2 μm sterile filtered 50 mM HEPES, 0.15 M NaCl, 0.05% DDM, 0.01% CHS, 6% Trehalose, pH 7.5
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.
  • 基因功能參考文獻(xiàn)：
    1. Authors report the generation of a human embryonic stem cell line WAe001-A-6 harbouring homozygous ASGR1 mutations using CRISPR/Cas9. The mutation involves a 37bp deletion, resulting in a frame shift. PMID: 28952928
    2. PEGylated lipoplexes were well tolerated by both HEK293 (ASGP-R-negative) and HepG2 (ASGP-R-positive) cell lines and delivered DNA to the hepatoma cell line HepG2 by ASGP-R mediation at levels three-fold greater than nonPEGylated lipoplexes. PMID: 28063265
    3. findings show that the hepatitis E virus (HEV) ORF2 protein interacts directly with the ectodomain of both ASGR1 and ASGR2; ASGPR is involved in and facilitates HEV infection by binding to ORF2 PMID: 27155063
    4. ASGR1 can inhibit the activity of V-ATPase by interacting with LASS2, thereby suppressing the metastatic potential of hepatoma cells. PMID: 27241665
    5. This review will focus on the mechanisms of platelet senescence with specific emphasis on the role of post-translational modifications in platelet life-span and thrombopoietin production downstream of the hepatic Ashwell-Morrell receptor, originally termed asialoglycoprotein receptor (ASGPR). PMID: 27207430
    6. ASGR1 haploinsufficiency was associated with reduced levels of non-HDL cholesterol and a reduced risk of coronary artery disease. PMID: 27192541
    7. Flow cytometric assessment of ASGPR expression may be a useful predictor of liver dysfunction following major hepatectomy for HCC in Chinese patients. PMID: 25404440
    8. Anti-ASGPR antibody could be used for specific and efficient HCC CTC enrichment, and anti-P-CK combined with anti-CPS1 antibodies is superior to identification with one antibody alone in the sensitivity for HCC CTC detection. PMID: 24763545
    9. Endocytic AMR controls TPO expression through Janus kinase 2 (JAK2) and the acute phase response signal transducer and activator of transcription 3 (STAT3) in vivo and in vitro PMID: 25485912
    10. Anti-ASGPR levels correlate with biochemical parameters and with the severity and manifestation autoimmune processes in patients with autoimmune hepatitis. PMID: 24933948
    11. The examined ASGPR1 expression levels by immunohistochemistry in HCC with different grades. Guidance for a targeting delivery strategy for anti-cancer drugs to HCC is suggested in this report. PMID: 23979840
    12. the distribution of ASGR in human testis, was investigated. PMID: 23604802
    13. the cooperative binding mode of Ca(2+) makes it possible for ASGP-R to be more sensitive to Ca(2+) concentrations in early endosomes, and plays an important role in the efficient release of ligand from ASGP-R PMID: 22613667
    14. Constant sH2a levels suggest constitutive secretion from hepatocytes in healthy individuals; therefore, a decrease with cirrhosis suggests a diagnostic potential. PMID: 22219600
    15. found that the asialoglycoprotein receptor (ASGPR) is involved in hepatocyte recognition of cells predestined for killing, including activated autologous T lymphocytes PMID: 21656538
    16. Asialoglycoprotein receptor (ASGPR) interacted specifically and directly with the preS1 domain of HBV in vivo and in vitro. PMID: 21207081
    17. Two naturally occurring ASGPR1 splice variants are produced in human hepatocytes. PMID: 20886072
    18. our findings suggest that both fibronectin and ASGPR mediate HBsAg binding to the cell surface, which provides further evidence for the potential roles of these two proteins in mediating HBV binding to liver cells. PMID: 20364278
    19. The minor subunit splice variants, H2b and H2c, of the human asialoglycoprotein receptor are present with the major subunit H1 in different hetero-oligomeric receptor complexes PMID: 11943787
    20. primary renal proximal tubular epithelial cells have a functional ASGPR, consisting of the H1 and H2 subunits, that is capable of specific ligand binding and uptake PMID: 12119473
    21. Phosphorylation-dependent interaction with molecular chaperones PMID: 12167617
    22. the effects of palmitoylation on ASGP-R activity and function PMID: 12359251
    23. the spacing of a Cys residue relative to the TMD in the primary protein sequence of H1 is the major determinant for successful palmitoylation PMID: 12370180
    24. Exposure of beta-galactose results in the rapid clearance of platelets from the circulation by asialoglycoprotein receptor-expressing liver macrophages and hepatocytes. PMID: 19520807

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  • 亞細(xì)胞定位：
    [Isoform H1a]: Membrane; Single-pass type II membrane protein.; [Isoform H1b]: Secreted.
  • 組織特異性：
    Expressed exclusively in hepatic parenchymal cells.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 742

    OMIM: 108360

    KEGG: hsa:432

    STRING: 9606.ENSP00000269299

    UniGene: Hs.12056