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  4. ATP6V1A Recombinant Monoclonal Antibody

ATP6V1A Recombinant Monoclonal Antibody

  • 中文名稱：
    ATP6V1A Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA193957A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Flow cytometric analysis of ATP6V1A expression in HepG2 cells using ATP6V1A antibody. Green, isotype control; red, ATP6V1A.
    • Immunocytochemical staining of HepG2 cells with ATP6V1A antibody. Nuclei were stained blue with DAPI; ATP6V1A was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
    • Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using ATP6V1A antibody. Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
    • Western blotting analysis using ATP6V1A antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP6V1A antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    ATP6V1A; ATPase H+ Transporting V1 Subunit A; V-ATPase Subunit A; ATP6V1A1; ATP6A1; Vma1; VA68; VPP2; ATPase, H+ Transporting, Lysosomal 70kDa, V1 Subunit A; V-Type Proton ATPase (V-ATPase) Catalytic Subunit A; V-Type Proton ATPase Catalytic Subunit A; Vacuolar Proton Pump Subunit Alpha; ATPase, H+ Transporting, Lysosomal (Vacuolar Proton Pump), Alpha Polypeptide, 70kD, Isoform 1; H+-Transporting ATPase Chain A, Vacuolar (VA68 Type); ATPase, H+ Transporting, Lysosomal, Subunit A1; H(+)-Transporting Two-Sector ATPase, Subunit A; Vacuolar Proton Pump Alpha Subunit 1; Vacuolar ATPase Isoform VA68; V-ATPase 69 KDa Subunit 1; Vacuolar-Type H(+)-ATPase; V-ATPase 69 KDa Subunit; V-ATPase A Subunit 1; EC 3.6.3.14; EC 7.1.2.2; EC 3.6.3; ARCL2D; IECEE3; DEE93; HO68
  • 反應種屬：
    Human, Mouse, Rat
  • 免疫原：
    Recombinant Human ATP6V1A protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    2D3
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, FC, ICC, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    FC 1:200-1:2000
    ICC 1:100-1:1000
    IHC 1:100-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation. May play a role in neurite development and synaptic connectivity.; (Microbial infection) Plays an important role in virion uncoating during Rabies virus replication after membrane fusion. Specifically, participates in the dissociation of incoming viral matrix M proteins uncoating through direct interaction.
  • 基因功能參考文獻：
    1. We report biallelic mutations in ATP6V1E1 and ATP6V1A, respectively encoding the E1 and A subunits of the V1 domain of V-ATPase, as a cause of distinct metabolic and multisystemic cutis laxa entities. PMID: 28065471
    2. V1A subunit of V-ATPase has a role in the progression and prognosis of gastric cancer. PMID: 25652905
    3. Data show that the cAMP/PKA/CREB signaling pathway initiates acidosis-induced V-ATPase trafficking in salivary ducts via regulation of Rab11b expression. PMID: 22561749
    4. These data introduce Rab11b as a crucial regulator and Rip11 as mediator of acidosis-induced V-ATPase traffic in duct cells of submandibular gland. PMID: 20717956
    5. there is an important role for physical association between aldolase and the A, B and E subunits of V-ATPase in the regulation of the proton pump PMID: 17576770

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  • 相關疾病：
    Cutis laxa, autosomal recessive, 2D (ARCL2D)
  • 亞細胞定位：
    Cytoplasm. Cytoplasmic vesicle, secretory vesicle.
  • 蛋白家族：
    ATPase alpha/beta chains family
  • 組織特異性：
    High expression in the skin.
  • 數據庫鏈接：

    HGNC: 851

    OMIM: 607027

    KEGG: hsa:523

    STRING: 9606.ENSP00000273398

    UniGene: Hs.477155