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  4. CTBP2 Recombinant Monoclonal Antibody

CTBP2 Recombinant Monoclonal Antibody

  • 中文名稱：
    CTBP2 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA257842A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      , Positive WB detected in:A549 whole cell lysate(30μg), HT29 whole cell lysate(30μg), COLO205 whole cell lysate(30μg), HELA whole cell lysate(30μg), U251 whole cell lysate(30μg), A431 whole cell lysate(30μg), Mouse brain tissue lysate(30μg), Rat brain lysate(30μg)
      All lanes: CtBP2 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 49 kDa
      Observed band size: 49 kDa
      Exposure time：10s
    • IHC image of CSB-RA257842A0HU diluted at 1:100 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA257842A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of HeLa cell with CSB-RA257842A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing Hela cells stained with CSB-RA257842A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    C terminal binding protein 2 antibody; C-terminal-binding protein 2 antibody; CtBP2 antibody; CTBP2_HUMAN antibody; ribeye antibody
  • 反應種屬：
    Human, Mouse, Rat
  • 免疫原：
    A synthesized peptide from human CTBP2 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    8D8
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation.; Isoform 2 probably acts as a scaffold for specialized synapses.
  • 基因功能參考文獻：
    1. the present study indicated that CtBP2 reduced the susceptibility of ECA109 cells to cisplatin by regulating the expression of apoptosis-related proteins, suggesting that it may be a promising therapeutic target in esophageal squamous cell carcinoma in the future. PMID: 29658564
    2. Results indicated that CTBP2 was direct target of miR-338-5p in glioma cells. CTBP2 silencing can rescued the phenotype changes induced by miR-338-5p inhibitor on cell proliferation and invasion in glioma. PMID: 28826173
    3. this study shows that CtBP2 overexpression promotes tumor cell proliferation and invasion in gastric cancer and is associated with poor prognosis PMID: 28404932
    4. these findings provide insight into the role CtBP2 plays in promoting proliferation and migration in breast cancer by the inhibition of p16INK4A. PMID: 28412731
    5. CtBP2 ameliorated palmitic acid-induced insulin resistance via ROS-dependent JNK pathway. PMID: 28111233
    6. CtBP2 is a druggable transforming oncoprotein critical for the evolution of neoplasia driven by Apc mutation. PMID: 28414304
    7. CtBP2 may be a potential target to suppress tumorigenesis in neuroblastoma PMID: 28179207
    8. This Gene-based tests suggest evidence of association with related genes, ZEB2, RND3, MCTP1, CTBP2, and beta EEG PMID: 28040410
    9. Our results indicated that CCNH/CDK7-CtBP2 axis may augment ESCC cell migration, and targeting the interaction of both may provide a novel therapeutic target of esophageal squamous cell carcinoma . PMID: 25820824
    10. Releasing the key adipogenic regulator C/EBPalpha from CtBP2 binding. PMID: 25895816
    11. Enhanced CtBP2expression promoted HCC xenograft growth and induced EMT. PMID: 25686837
    12. results show how CtBP2 contributes to prostate cancer progression by modulating AR and oncogenic signaling PMID: 25228652
    13. High CTBP2 expression is associated with prostate cancer. PMID: 24332637
    14. Overexpression of CtBP2 is associated with breast carcinoma. PMID: 24522810
    15. CtBP2 might contribute to the progression of esophageal squamous cell carcinoma through a negative transcriptional regulation of p16(INK4A). PMID: 23255392
    16. CtBP2 is over-expressed in prostate cancer.CtBP2 promotes prostate cancer cell proliferation through c-Myc signaling. PMID: 24835310
    17. BRCA1 expression is epigenetically repressed in sporadic ovarian cancer cells by overexpression of C-terminal binding protein 2.CtBP2 is an ovarian cancer oncogene. PMID: 23730208
    18. CtBP2 directly targeted stem cell core genes resulting in increased cancer cell stemness and increasing metastatic and tumorigenic potential. PMID: 24012420
    19. E2F7 recruits the co-repressor C-terminal-binding protein (CtBP) and that CtBP2 is essential for E2F7 to repress E2F1 transcription. PMID: 23853115
    20. CTBP2 is a transcriptional cofactor for RXR-alpha/RAR-alpha. PMID: 23775127
    21. We propose that CtBP2 is an ovarian cancer oncogene that regulates gene expression program by modulating HDAC activity. PMID: 22945647
    22. these data demonstrate that CHIP regulates the steady-state level of CtBP2 as an E3 ubiquitin ligase and determines the expression levels of CtBP2 target genes. PMID: 23410750
    23. Interaction with cyclin H/cyclin-dependent kinase 7 (CCNH/CDK7) stabilizes C-terminal binding protein 2 (CtBP2) and promotes cancer cell migration PMID: 23393140
    24. Doubly transgenic zebrafish exhibit a startle response and typical swimming behavior, indicating that there is no gross disruption of either hearing or vestibular function useful in the study of ribbon synapse development of the hair cell. PMID: 21334379
    25. This study demonstrates that ataxin-1 occupies the promoter region of E-cadherin in vivo and that ataxin-1 activates the promoter in a CtBP2-mediated transcriptional regulation manner. PMID: 21315774
    26. we demonstrate that it is the interaction of CtBPs with transcriptional regulators and/or chromatin-modifying enzymes in the cell nucleus, rather than their role in Golgi fission, which is critical for the maintenance of mitotic fidelity. PMID: 21057548
    27. CtBP2 proteins are ubiquitously expressed in all lines and tumour samples. PMID: 20964627
    28. CtBP2 selectively down-regulates Th2 cytokines, therefore it is a potential target for the treatment of allergic diseases. PMID: 20523059
    29. Data suggest that ARF antagonism of CtBP repression of Bik and other BH3-only genes may have a critical role in ARF-induced p53-independent apoptosis and tumor suppression. PMID: 19798104
    30. roles of acetylation in regulating subcellular localization and transcriptional activity of CtBP2. PMID: 16356938
    31. analysis of the CtBP2 corepressor complex induced by E1A and modulation of E1A transcriptional activity by CtBP2 PMID: 17023432
    32. E1A may gain access to cellular promoters through conservved sequence-dependent interaction with CtBP2. PMID: 17546044
    33. Study shows that transcription corepressor CtBP2 directly binds acinus, which is regulated by nerve growth factor (NGF), inhibiting its stimulatory effect on cyclin A1, but not cyclin A2, expression in leukemia. PMID: 19668232
    34. CtBP2 monomer interacts with a major CtBP-dependent repressor ZEB and HDAC and that the interaction of the two factors with the CtBP2 monomer was mutually exclusive. PMID: 19754958
    35. Genome-wide association study of gene-disease association. (HuGE Navigator) PMID: 18264096

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  • 亞細胞定位：
    Nucleus. Cell junction, synapse.
  • 蛋白家族：
    D-isomer specific 2-hydroxyacid dehydrogenase family
  • 組織特異性：
    Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas.
  • 數據庫鏈接：

    HGNC: 2495

    OMIM: 602619

    KEGG: hsa:1488

    STRING: 9606.ENSP00000311825

    UniGene: Hs.501345