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  4. CUL2 Recombinant Monoclonal Antibody

CUL2 Recombinant Monoclonal Antibody

  • 中文名稱：
    CUL2 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA221739A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Flow cytometric analysis of Cullin 2 expression in HepG2 cells using Cullin 2 antibody. Green, isotype control; red, Cullin 2.
    • Immunocytochemical staining of HepG2 cells with cullin 2 antibody. Nuclei were stained blue with DAPI; Cullin 2 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
    • Western blotting analysis using Cullin 2 antibody. Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Cullin 2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    CUL2; Cullin 2; Cullin-2; CUL-2; Testis Secretory Sperm-Binding Protein Li 238E
  • 反應種屬：
    Human, Mouse, Rat
  • 免疫原：
    Recombinant Human CUL2 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    13A6
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, FC, ICC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    FC 1:200-1:2000
    ICC 1:100-1:1000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. CUL2 may serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF). A number of ECS complexes (containing either KLHDC2, KLHDC3, KLHDC10, APPBP2, FEM1A, FEM1B or FEM1C as substrate-recognition component) are part of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins.
  • 基因功能參考文獻：
    1. direct evidence that both A3A and HPV16 E7 interact with CUL2, suggesting that the E7-CUL2 complex formed during HPV infection may regulate A3A protein levels in the cell. PMID: 29367246
    2. The crystal structure of a pentameric CRL2(VHL) complex, composed of Cul2, Rbx1, Elongin B, Elongin C, and pVHL reveals hydrophobic contact residues critical for the Cul2 interactions. PMID: 28591624
    3. Results suggest that E7 recruited CUL2, driven by CUL2/E2F1/miR-424 regulatory loop, is overexpressed and accelerates HPV16-induced cervical carcinogenesis. PMID: 27153550
    4. Cullins are a family of NEDD8 targets important in the stabilization and degradation of proteins, such as hypoxia-inducible factor (HIF1A), via Cullin-2. PMID: 25326537
    5. Nevertheless, the identification of the Cul2 box may allow prediction of Cullin specificity for all E4orf6-containing Adenoviridae. PMID: 24453364
    6. a novel link between the oncoprotein PRAME and the conserved EKC complex PMID: 22912744
    7. Study finds that UBXN7 over-expression converts CUL2 to its neddylated form and causes the accumulation of non-ubiquitylated HIF1alpha. PMID: 22537386
    8. The authors applied protein-complex purification strategies and identified PRAME as a substrate recognition subunit of a Cullin2-based E3 ubiquitin ligase. PMID: 21822215
    9. The data suggest that neither HIF1alpha nor CUL2 mutation may play a central role in HIF1alpha activation in gastric, colorectal, breast, lung and hepatocellular carcinomas, and acute leukemias. PMID: 20078552
    10. Cullin selection is determined by specific Elongin C and Skp1 sequences PMID: 15280393
    11. the HPV16 E7-associated cullin 2 ubiquitin ligase complex contributes to aberrant degradation of the pRB tumor suppressor in HPV16 E7-expressing cells. PMID: 17609271
    12. CUL2 is required for normal vasculogenesis, at least in part mediated by its regulation of HIF-mediated transcription. PMID: 18372249

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  • 蛋白家族：
    Cullin family
  • 數據庫鏈接：

    HGNC: 2552

    OMIM: 603135

    KEGG: hsa:8453

    STRING: 9606.ENSP00000444856

    UniGene: Hs.82919