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PRKAG1 Recombinant Monoclonal Antibody

  • 中文名稱：
    PRKAG1重組抗體
  • 貨號(hào)：
    CSB-RA161743A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • IHC image of CSB-RA161743A0HU diluted at 1:100 and staining in paraffin-embedded human Prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA161743A0HU PRKAG1重組單克隆抗體是針對(duì)AMP激活蛋白激酶γ1亞基（PRKAG1）研發(fā)的高特異性科研工具。該抗體靶向的PRKAG1作為AMPK復(fù)合物的關(guān)鍵調(diào)控組分，在細(xì)胞能量代謝平衡、糖脂代謝調(diào)控及應(yīng)激響應(yīng)中發(fā)揮核心作用，其功能異常與代謝性疾病及心血管疾病的發(fā)生發(fā)展密切相關(guān)。本產(chǎn)品經(jīng)嚴(yán)格驗(yàn)證，在ELISA和免疫組化（IHC）實(shí)驗(yàn)中展現(xiàn)優(yōu)異性能，特別適用于石蠟包埋組織樣本的檢測(cè)，推薦使用稀釋比例為1:50-1:200時(shí)可獲得清晰的特異性信號(hào)。該抗體適用于探究能量代謝相關(guān)信號(hào)通路機(jī)制、疾病模型中的蛋白表達(dá)定位研究，以及代謝綜合征、心肌肥厚等病理過程的分子機(jī)制解析，為細(xì)胞代謝調(diào)控、疾病生物標(biāo)志物篩選等基礎(chǔ)研究領(lǐng)域提供可靠檢測(cè)方案。
  • Uniprot No.：
  • 基因名：
  • 別名：
    5'-AMP-activated protein kinase subunit gamma-1 (AMPK gamma1) (AMPK subunit gamma-1) (AMPKg), PRKAG1
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human PRKAG1
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    5E5
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    IHC 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.
  • 基因功能參考文獻(xiàn)：
    1. Data suggest different gamma-isoforms in AMPK can have different effects on enzyme activation; here, activation of AMPK by compound 991 is greater if AMPK contains PRKAG2 versus PRKAG1 or PRKAG3. PMID: 28302767
    2. Pin1 plays an important role in the pathogenic mechanisms underlying impaired glucose and lipid metabolism, functioning as a negative regulator of AMPK PMID: 26276391
    3. Letter: report PRKAG1 frameshift mutations in colorectal cancer. PMID: 25267969
    4. Electrogenic phosphate transport in sodium/phosphate cotransporter NaPi-IIa-expressing Xenopus oocytes is markedly decreased by the coexpression of constitutively active AMPKgammaR70Q, but not of AMPK(alphaK45R). PMID: 24356547
    5. PRKAG1 interacts with DSCAM through its gamma subunit, and netrin-1 activates AMPK phosphorylation in cortical neurons. PMID: 23479427
    6. Conclude that cTnI phosphorylation by AMPK may represent a novel mechanism of regulation of cardiac function. PMID: 22456184
    7. Inhibition of the KCa3.1 channels by AMP-activated protein kinase in human airway epithelial cells. PMID: 19052260

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  • 蛋白家族：
    5'-AMP-activated protein kinase gamma subunit family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 9385

    OMIM: 602742

    KEGG: hsa:5571

    STRING: 9606.ENSP00000323867

    UniGene: Hs.530862