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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia phytofirmans 50S ribosomal protein L27 (rpmA) CSB-YP454788BXU
CSB-EP454788BXU
CSB-BP454788BXU
CSB-MP454788BXU
CSB-EP454788BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD), partial CSB-YP454789BXU
CSB-EP454789BXU
CSB-BP454789BXU
CSB-MP454789BXU
CSB-EP454789BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Sec-independent protein translocase protein TatA (tatA) CSB-YP454790BXU
CSB-EP454790BXU
CSB-BP454790BXU
CSB-MP454790BXU
CSB-EP454790BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 50S ribosomal protein L35 (rpmI) CSB-YP454791BXU
CSB-EP454791BXU
CSB-BP454791BXU
CSB-MP454791BXU
CSB-EP454791BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP454792BXU
CSB-EP454792BXU
CSB-BP454792BXU
CSB-MP454792BXU
CSB-EP454792BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Urease subunit beta (ureB) CSB-YP454793BXU
CSB-EP454793BXU
CSB-BP454793BXU
CSB-MP454793BXU
CSB-EP454793BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP454794BXU
CSB-EP454794BXU
CSB-BP454794BXU
CSB-MP454794BXU
CSB-EP454794BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans S-adenosylmethionine synthase (metK) CSB-YP454795BXU
CSB-EP454795BXU
CSB-BP454795BXU
CSB-MP454795BXU
CSB-EP454795BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP454796BXU
CSB-EP454796BXU
CSB-BP454796BXU
CSB-MP454796BXU
CSB-EP454796BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Ribose-5-phosphate isomerase A (rpiA) CSB-YP454797BXU
CSB-EP454797BXU
CSB-BP454797BXU
CSB-MP454797BXU
CSB-EP454797BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans UPF0303 protein Bphyt_1734 (Bphyt_1734) CSB-YP454798BXU
CSB-EP454798BXU
CSB-BP454798BXU
CSB-MP454798BXU
CSB-EP454798BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Probable intracellular septation protein A (Bphyt_1922), partial CSB-YP454799BXU1
CSB-EP454799BXU1
CSB-BP454799BXU1
CSB-MP454799BXU1
CSB-EP454799BXU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Ferredoxin--NADP reductase (Bphyt_2243) CSB-YP454800BXU
CSB-EP454800BXU
CSB-BP454800BXU
CSB-MP454800BXU
CSB-EP454800BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Lipid-A-disaccharide synthase (lpxB) CSB-YP454801BXU
CSB-EP454801BXU
CSB-BP454801BXU
CSB-MP454801BXU
CSB-EP454801BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 30S ribosomal protein S2 (rpsB) CSB-YP454802BXU
CSB-EP454802BXU
CSB-BP454802BXU
CSB-MP454802BXU
CSB-EP454802BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Bifunctional protein FolD (folD) CSB-YP454803BXU
CSB-EP454803BXU
CSB-BP454803BXU
CSB-MP454803BXU
CSB-EP454803BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans tRNA (guanine-N (1)-)-methyltransferase CSB-YP454804BXU
CSB-EP454804BXU
CSB-BP454804BXU
CSB-MP454804BXU
CSB-EP454804BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP454805BXU
CSB-EP454805BXU
CSB-BP454805BXU
CSB-MP454805BXU
CSB-EP454805BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Adenine deaminase (Bphyt_3044) CSB-YP454806BXU
CSB-EP454806BXU
CSB-BP454806BXU
CSB-MP454806BXU
CSB-EP454806BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans N utilization substance protein B homolog (nusB) CSB-YP454807BXU
CSB-EP454807BXU
CSB-BP454807BXU
CSB-MP454807BXU
CSB-EP454807BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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