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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus carnosus Probable DNA-directed RNA polymerase subunit delta (rpoE) CSB-YP494962FLK
CSB-EP494962FLK
CSB-BP494962FLK
CSB-MP494962FLK
CSB-EP494962FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus ATP synthase subunit c (atpE), partial CSB-YP494963FLK1
CSB-EP494963FLK1
CSB-BP494963FLK1
CSB-MP494963FLK1
CSB-EP494963FLK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Protein sprT-like (Sca_1568) CSB-YP494964FLK
CSB-EP494964FLK
CSB-BP494964FLK
CSB-MP494964FLK
CSB-EP494964FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP494965FLK
CSB-EP494965FLK
CSB-BP494965FLK
CSB-MP494965FLK
CSB-EP494965FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus DNA polymerase IV (dinB) CSB-YP494966FLK
CSB-EP494966FLK
CSB-BP494966FLK
CSB-MP494966FLK
CSB-EP494966FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus UPF0374 protein Sca_1440 (Sca_1440) CSB-YP494967FLK
CSB-EP494967FLK
CSB-BP494967FLK
CSB-MP494967FLK
CSB-EP494967FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP494968FLK
CSB-EP494968FLK
CSB-BP494968FLK
CSB-MP494968FLK
CSB-EP494968FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Formate--tetrahydrofolate ligase (fhs), partial CSB-YP494969FLK
CSB-EP494969FLK
CSB-BP494969FLK
CSB-MP494969FLK
CSB-EP494969FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Transcriptional repressor NrdR (nrdR) CSB-YP494970FLK
CSB-EP494970FLK
CSB-BP494970FLK
CSB-MP494970FLK
CSB-EP494970FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP494971FLK
CSB-EP494971FLK
CSB-BP494971FLK
CSB-MP494971FLK
CSB-EP494971FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus 50S ribosomal protein L27 (rpmA) CSB-YP494972FLK
CSB-EP494972FLK
CSB-BP494972FLK
CSB-MP494972FLK
CSB-EP494972FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Putative Holliday junction resolvase (Sca_1228) CSB-YP494973FLK
CSB-EP494973FLK
CSB-BP494973FLK
CSB-MP494973FLK
CSB-EP494973FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Chaperone protein DnaK (dnaK), partial CSB-YP494974FLK
CSB-EP494974FLK
CSB-BP494974FLK
CSB-MP494974FLK
CSB-EP494974FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Glycine--tRNA ligase (glyQS) CSB-YP494975FLK
CSB-EP494975FLK
CSB-BP494975FLK
CSB-MP494975FLK
CSB-EP494975FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP494976FLK
CSB-EP494976FLK
CSB-BP494976FLK
CSB-MP494976FLK
CSB-EP494976FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Segregation and condensation protein A (scpA) CSB-YP494977FLK
CSB-EP494977FLK
CSB-BP494977FLK
CSB-MP494977FLK
CSB-EP494977FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Demethylmenaquinone methyltransferase (ubiE) CSB-YP494978FLK
CSB-EP494978FLK
CSB-BP494978FLK
CSB-MP494978FLK
CSB-EP494978FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Dihydrodipicolinate reductase (dapB) CSB-YP494979FLK
CSB-EP494979FLK
CSB-BP494979FLK
CSB-MP494979FLK
CSB-EP494979FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Homoserine kinase (thrB) CSB-YP494980FLK
CSB-EP494980FLK
CSB-BP494980FLK
CSB-MP494980FLK
CSB-EP494980FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP494981FLK
CSB-EP494981FLK
CSB-BP494981FLK
CSB-MP494981FLK
CSB-EP494981FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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