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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Uncharacterized ABC transporter ATP-binding protein YknU (yknU), partial CSB-YP521756BRJ1
CSB-EP521756BRJ1
CSB-BP521756BRJ1
CSB-MP521756BRJ1
CSB-EP521756BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized ABC transporter permease yknZ (yknZ), partial CSB-YP521757BRJ1
CSB-EP521757BRJ1
CSB-BP521757BRJ1
CSB-MP521757BRJ1
CSB-EP521757BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized oxidoreductase ykpB (ykpB) CSB-YP521758BRJ
CSB-EP521758BRJ
CSB-BP521758BRJ
CSB-MP521758BRJ
CSB-EP521758BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis N-formyl-4-amino-5-aminomethyl-2-methylpyrimidine deformylase (ylmB) CSB-YP521759BRJ
CSB-EP521759BRJ
CSB-BP521759BRJ
CSB-MP521759BRJ
CSB-EP521759BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Cell division protein SepF (sepF) CSB-YP521760BRJ
CSB-EP521760BRJ
CSB-BP521760BRJ
CSB-MP521760BRJ
CSB-EP521760BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Ribosome maturation factor RimM (rimM) CSB-YP521761BRJ
CSB-EP521761BRJ
CSB-BP521761BRJ
CSB-MP521761BRJ
CSB-EP521761BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ylqG (ylqG), partial CSB-YP521762BRJ
CSB-EP521762BRJ
CSB-BP521762BRJ
CSB-MP521762BRJ
CSB-EP521762BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Zinc metalloprotease rasP (rasP), partial CSB-YP521763BRJ1
CSB-EP521763BRJ1
CSB-BP521763BRJ1
CSB-MP521763BRJ1
CSB-EP521763BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable inactive metalloprotease ymfF (ymfF) CSB-YP521764BRJ
CSB-EP521764BRJ
CSB-BP521764BRJ
CSB-MP521764BRJ
CSB-EP521764BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein ymfM (ymfM), partial CSB-YP521765BRJ1
CSB-EP521765BRJ1
CSB-BP521765BRJ1
CSB-MP521765BRJ1
CSB-EP521765BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative 8-amino-7-oxononanoate synthase/2-amino-3-ketobutyrate coenzyme A ligase (kbl) CSB-YP521766BRJ
CSB-EP521766BRJ
CSB-BP521766BRJ
CSB-MP521766BRJ
CSB-EP521766BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Polyketide synthase PksR (pksR), partial CSB-YP521767BRJ
CSB-EP521767BRJ
CSB-BP521767BRJ
CSB-MP521767BRJ
CSB-EP521767BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0714 protein ymaC (ymaC), partial CSB-YP521768BRJ
CSB-EP521768BRJ
CSB-BP521768BRJ
CSB-MP521768BRJ
CSB-EP521768BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Protein hfq (hfq) CSB-YP521769BRJ
CSB-EP521769BRJ
CSB-BP521769BRJ
CSB-MP521769BRJ
CSB-EP521769BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable deoxyuridine 5'-triphosphate nucleotidohydrolase yncF (yncF) CSB-YP521770BRJ
CSB-EP521770BRJ
CSB-BP521770BRJ
CSB-MP521770BRJ
CSB-EP521770BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein YndB (yndB) CSB-YP521771BRJ
CSB-EP521771BRJ
CSB-BP521771BRJ
CSB-MP521771BRJ
CSB-EP521771BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yndH (yndH) CSB-YP521772BRJ
CSB-EP521772BRJ
CSB-BP521772BRJ
CSB-MP521772BRJ
CSB-EP521772BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein yndK (yndK), partial CSB-YP521773BRJ1
CSB-EP521773BRJ1
CSB-BP521773BRJ1
CSB-MP521773BRJ1
CSB-EP521773BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Thioredoxin-like protein yneN (yneN), partial CSB-YP521774BRJ
CSB-EP521774BRJ
CSB-BP521774BRJ
CSB-MP521774BRJ
CSB-EP521774BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative acyl-CoA synthetase YngI (yngI), partial CSB-YP521775BRJ
CSB-EP521775BRJ
CSB-BP521775BRJ
CSB-MP521775BRJ
CSB-EP521775BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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