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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Streptomyces griseus subsp. griseus Ketol-acid reductoisomerase (ilvC) CSB-YP541566FOF
CSB-EP541566FOF
CSB-BP541566FOF
CSB-MP541566FOF
CSB-EP541566FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP541567FOF
CSB-EP541567FOF
CSB-BP541567FOF
CSB-MP541567FOF
CSB-EP541567FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 50S ribosomal protein L31 (rpmE) CSB-YP541568FOF
CSB-EP541568FOF
CSB-BP541568FOF
CSB-MP541568FOF
CSB-EP541568FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus UvrABC system protein C (uvrC), partial CSB-YP541569FOF
CSB-EP541569FOF
CSB-BP541569FOF
CSB-MP541569FOF
CSB-EP541569FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Cytidylate kinase (cmk) CSB-YP541570FOF
CSB-EP541570FOF
CSB-BP541570FOF
CSB-MP541570FOF
CSB-EP541570FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP541571FOF
CSB-EP541571FOF
CSB-BP541571FOF
CSB-MP541571FOF
CSB-EP541571FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Proteasome subunit beta (prcB) CSB-YP541572FOF
CSB-EP541572FOF
CSB-BP541572FOF
CSB-MP541572FOF
CSB-EP541572FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 50S ribosomal protein L20 (rplT) CSB-YP541573FOF
CSB-EP541573FOF
CSB-BP541573FOF
CSB-MP541573FOF
CSB-EP541573FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP541574FOF
CSB-EP541574FOF
CSB-BP541574FOF
CSB-MP541574FOF
CSB-EP541574FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Bifunctional protein FolD (folD) CSB-YP541575FOF
CSB-EP541575FOF
CSB-BP541575FOF
CSB-MP541575FOF
CSB-EP541575FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 30S ribosomal protein S5 (rpsE) CSB-YP541576FOF
CSB-EP541576FOF
CSB-BP541576FOF
CSB-MP541576FOF
CSB-EP541576FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 50S ribosomal protein L5 (rplE) CSB-YP541577FOF
CSB-EP541577FOF
CSB-BP541577FOF
CSB-MP541577FOF
CSB-EP541577FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 50S ribosomal protein L22 (rplV) CSB-YP541578FOF
CSB-EP541578FOF
CSB-BP541578FOF
CSB-MP541578FOF
CSB-EP541578FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Elongation factor P (efp) CSB-YP541579FOF
CSB-EP541579FOF
CSB-BP541579FOF
CSB-MP541579FOF
CSB-EP541579FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP541580FOF
CSB-EP541580FOF
CSB-BP541580FOF
CSB-MP541580FOF
CSB-EP541580FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP541581FOF
CSB-EP541581FOF
CSB-BP541581FOF
CSB-MP541581FOF
CSB-EP541581FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus NADH-quinone oxidoreductase subunit K 2 (nuoK2), partial CSB-YP541582FOF1
CSB-EP541582FOF1
CSB-BP541582FOF1
CSB-MP541582FOF1
CSB-EP541582FOF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Urease subunit gamma (ureA) CSB-YP541583FOF
CSB-EP541583FOF
CSB-BP541583FOF
CSB-MP541583FOF
CSB-EP541583FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus UPF0264 protein SGR_6403 (SGR_6403) CSB-YP541584FOF
CSB-EP541584FOF
CSB-BP541584FOF
CSB-MP541584FOF
CSB-EP541584FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Poly (U)-specific endoribonuclease-B CSB-YP541585XBE
CSB-EP541585XBE
CSB-BP541585XBE
CSB-MP541585XBE
CSB-EP541585XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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