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重組蛋白

產品名稱 貨號 規格
Recombinant Ralstonia metallidurans 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP634769RJG
CSB-EP634769RJG
CSB-BP634769RJG
CSB-MP634769RJG
CSB-EP634769RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Glucose-6-phosphate isomerase (pgi), partial CSB-YP634770RJG
CSB-EP634770RJG
CSB-BP634770RJG
CSB-MP634770RJG
CSB-EP634770RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Ribonuclease HII (rnhB) CSB-YP634771RJG
CSB-EP634771RJG
CSB-BP634771RJG
CSB-MP634771RJG
CSB-EP634771RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Uridylate kinase (pyrH) CSB-YP634772RJG
CSB-EP634772RJG
CSB-BP634772RJG
CSB-MP634772RJG
CSB-EP634772RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Disulfide bond formation protein B (dsbB), partial CSB-YP634773RJG1
CSB-EP634773RJG1
CSB-BP634773RJG1
CSB-MP634773RJG1
CSB-EP634773RJG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP634774RJG
CSB-EP634774RJG
CSB-BP634774RJG
CSB-MP634774RJG
CSB-EP634774RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Enolase 2 (eno2) CSB-YP634775RJG
CSB-EP634775RJG
CSB-BP634775RJG
CSB-MP634775RJG
CSB-EP634775RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Cell division protein FtsB (ftsB), partial CSB-YP634776RJG
CSB-EP634776RJG
CSB-BP634776RJG
CSB-MP634776RJG
CSB-EP634776RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Ferrochelatase (hemH) CSB-YP634777RJG
CSB-EP634777RJG
CSB-BP634777RJG
CSB-MP634777RJG
CSB-EP634777RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans 2-isopropylmalate synthase (leuA), partial CSB-YP634778RJG
CSB-EP634778RJG
CSB-BP634778RJG
CSB-MP634778RJG
CSB-EP634778RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans PKHD-type hydroxylase Rmet_0838 (Rmet_0838) CSB-YP634779RJG
CSB-EP634779RJG
CSB-BP634779RJG
CSB-MP634779RJG
CSB-EP634779RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Lipid A export ATP-binding/permease protein MsbA (msbA), partial CSB-YP634780RJG1
CSB-EP634780RJG1
CSB-BP634780RJG1
CSB-MP634780RJG1
CSB-EP634780RJG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Cytidylate kinase (cmk) CSB-YP634781RJG
CSB-EP634781RJG
CSB-BP634781RJG
CSB-MP634781RJG
CSB-EP634781RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans UPF0434 protein Rmet_0534 (Rmet_0534) CSB-YP634782RJG
CSB-EP634782RJG
CSB-BP634782RJG
CSB-MP634782RJG
CSB-EP634782RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP634783RJG
CSB-EP634783RJG
CSB-BP634783RJG
CSB-MP634783RJG
CSB-EP634783RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans D-tyrosyl-tRNA (Tyr) deacylase (dtd) CSB-YP634784RJG
CSB-EP634784RJG
CSB-BP634784RJG
CSB-MP634784RJG
CSB-EP634784RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP634785RJG
CSB-EP634785RJG
CSB-BP634785RJG
CSB-MP634785RJG
CSB-EP634785RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Phosphopantetheine adenylyltransferase (coaD) CSB-YP634786RJG
CSB-EP634786RJG
CSB-BP634786RJG
CSB-MP634786RJG
CSB-EP634786RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Multifunctional CCA protein (cca) CSB-YP634787RJG
CSB-EP634787RJG
CSB-BP634787RJG
CSB-MP634787RJG
CSB-EP634787RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Acetylglutamate kinase (argB) CSB-YP634788RJG
CSB-EP634788RJG
CSB-BP634788RJG
CSB-MP634788RJG
CSB-EP634788RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業產品。重組蛋白的技術資源在重組蛋白的開發過程中,除了表達系統之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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