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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ralstonia metallidurans tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP637994RJG
CSB-EP637994RJG
CSB-BP637994RJG
CSB-MP637994RJG
CSB-EP637994RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans 30S ribosomal protein S20 (rpsT) CSB-YP637995RJG
CSB-EP637995RJG
CSB-BP637995RJG
CSB-MP637995RJG
CSB-EP637995RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans 50S ribosomal protein L28 (rpmB) CSB-YP637996RJG
CSB-EP637996RJG
CSB-BP637996RJG
CSB-MP637996RJG
CSB-EP637996RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans N utilization substance protein B homolog (nusB) CSB-YP637997RJG
CSB-EP637997RJG
CSB-BP637997RJG
CSB-MP637997RJG
CSB-EP637997RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP637998RJG
CSB-EP637998RJG
CSB-BP637998RJG
CSB-MP637998RJG
CSB-EP637998RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Malate dehydrogenase (mdh) CSB-YP637999RJG
CSB-EP637999RJG
CSB-BP637999RJG
CSB-MP637999RJG
CSB-EP637999RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans 50S ribosomal protein L32 (rpmF) CSB-YP638000RJG
CSB-EP638000RJG
CSB-BP638000RJG
CSB-MP638000RJG
CSB-EP638000RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP638001RJG
CSB-EP638001RJG
CSB-BP638001RJG
CSB-MP638001RJG
CSB-EP638001RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Phosphate import ATP-binding protein PstB (pstB) CSB-YP638002RJG
CSB-EP638002RJG
CSB-BP638002RJG
CSB-MP638002RJG
CSB-EP638002RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Protein hfq (hfq) CSB-YP638003RJG
CSB-EP638003RJG
CSB-BP638003RJG
CSB-MP638003RJG
CSB-EP638003RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans sn-glycerol-3-phosphate import ATP-binding protein UgpC (ugpC) CSB-YP638004RJG
CSB-EP638004RJG
CSB-BP638004RJG
CSB-MP638004RJG
CSB-EP638004RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans 30S ribosomal protein S6 (rpsF) CSB-YP638005RJG
CSB-EP638005RJG
CSB-BP638005RJG
CSB-MP638005RJG
CSB-EP638005RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Thymidylate kinase (tmk) CSB-YP638006RJG
CSB-EP638006RJG
CSB-BP638006RJG
CSB-MP638006RJG
CSB-EP638006RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Ribulose bisphosphate carboxylase large chain (cbbL) CSB-YP638007RJG
CSB-EP638007RJG
CSB-BP638007RJG
CSB-MP638007RJG
CSB-EP638007RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Ribosome-recycling factor (frr) CSB-YP638008RJG
CSB-EP638008RJG
CSB-BP638008RJG
CSB-MP638008RJG
CSB-EP638008RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Alanine racemase (alr) CSB-YP638009RJG
CSB-EP638009RJG
CSB-BP638009RJG
CSB-MP638009RJG
CSB-EP638009RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Putative arginyl-tRNA--protein transferase (ate) CSB-YP638010RJG
CSB-EP638010RJG
CSB-BP638010RJG
CSB-MP638010RJG
CSB-EP638010RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans 50S ribosomal protein L20 (rplT) CSB-YP638011RJG
CSB-EP638011RJG
CSB-BP638011RJG
CSB-MP638011RJG
CSB-EP638011RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Lysine--tRNA ligase (lysS), partial CSB-YP638012RJG
CSB-EP638012RJG
CSB-BP638012RJG
CSB-MP638012RJG
CSB-EP638012RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Urease accessory protein UreF (ureF) CSB-YP638013RJG
CSB-EP638013RJG
CSB-BP638013RJG
CSB-MP638013RJG
CSB-EP638013RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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