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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Superoxide dismutase [Mn] (sodA) CSB-YP022398MVJ
CSB-EP022398MVJ
CSB-BP022398MVJ
CSB-MP022398MVJ
CSB-EP022398MVJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crocidura russula Acetylcholine receptor subunit alpha (CHRNA1) CSB-YP005386CSI
CSB-EP005386CSI
CSB-BP005386CSI
CSB-MP005386CSI
CSB-EP005386CSI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Voltage-dependent P/Q-type calcium channel subunit alpha-1A (Cacna1a), partial CSB-YP004397RA
CSB-EP004397RA
CSB-BP004397RA
CSB-MP004397RA
CSB-EP004397RA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Septin-2 (Sep2) CSB-YP021025DLU
CSB-EP021025DLU
CSB-BP021025DLU
CSB-MP021025DLU
CSB-EP021025DLU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Homeobox protein goosecoid (Gsc) CSB-YP009950DLU
CSB-EP009950DLU
CSB-BP009950DLU
CSB-MP009950DLU
CSB-EP009950DLU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avena sativa T-complex protein 1 subunit epsilon, partial CSB-YP004862DPO
CSB-EP004862DPO
CSB-BP004862DPO
CSB-MP004862DPO
CSB-EP004862DPO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Glucokinase (glcK) CSB-YP009319BRJ
CSB-EP009319BRJ
CSB-BP009319BRJ
CSB-MP009319BRJ
CSB-EP009319BRJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Branched-chain-amino-acid aminotransferase (ilvE) CSB-YP002601HTA
CSB-EP002601HTA
CSB-BP002601HTA
CSB-MP002601HTA
CSB-EP002601HTA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae UBX domain-containing protein 4 (UBX4) CSB-YP025536SVG
CSB-EP025536SVG
CSB-BP025536SVG
CSB-MP025536SVG
CSB-EP025536SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ustilago maydis Dolichol-phosphate mannosyltransferase (DPM1), partial CSB-YP007134UBS1
CSB-EP007134UBS1
CSB-BP007134UBS1
CSB-MP007134UBS1
CSB-EP007134UBS1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Hydroxymethylglutaryl-CoA synthase (HMGS) CSB-YP010567DOA
CSB-EP010567DOA
CSB-BP010567DOA
CSB-MP010567DOA
CSB-EP010567DOA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Geranylgeranyl pyrophosphate synthase (crtE) CSB-YP009393RLF
CSB-EP009393RLF
CSB-BP009393RLF
CSB-MP009393RLF
CSB-EP009393RLF-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heliothis virescens V-type proton ATPase 16 kDa proteolipid subunit (VHA16) CSB-YP002389HVF
CSB-EP002389HVF
CSB-BP002389HVF
CSB-MP002389HVF
CSB-EP002389HVF-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Complement component C8 beta chain (C8b) CSB-YP004195RA
CSB-EP004195RA
CSB-BP004195RA
CSB-MP004195RA
CSB-EP004195RA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Cysteine desulfurase (nifS) CSB-YP015769RKX
CSB-EP015769RKX
CSB-BP015769RKX
CSB-MP015769RKX
CSB-EP015769RKX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gadus morhua NADH-ubiquinone oxidoreductase chain 5 (MT-ND5), partial CSB-YP015081GER
CSB-EP015081GER
CSB-BP015081GER
CSB-MP015081GER
CSB-EP015081GER-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Horse 40S ribosomal protein S4 (RPS4) CSB-YP020458HO
CSB-EP020458HO
CSB-BP020458HO
CSB-MP020458HO
CSB-EP020458HO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Paired box protein Pax-6 (pax6) CSB-YP017492XBE
CSB-EP017492XBE
CSB-BP017492XBE
CSB-MP017492XBE
CSB-EP017492XBE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Caspase-3 (casp3) CSB-YP004548XBE
CSB-EP004548XBE
CSB-BP004548XBE
CSB-MP004548XBE
CSB-EP004548XBE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans Electron transfer flavoprotein-ubiquinone oxidoreductase CSB-YP007846PBZ
CSB-BP007846PBZ
CSB-MP007846PBZ
CSB-EP007846PBZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中，除了表達系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達載體?

表達載體是一種DNA分子，它攜帶特定的基因進入宿主細胞，并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達，經(jīng)過多年的發(fā)展，表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條：如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.jhhuazhong.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達服務，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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