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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vibrio vulnificus ATP-dependent RNA helicase rhlB (rhlB) CSB-YP745415VCQ
CSB-EP745415VCQ
CSB-BP745415VCQ
CSB-MP745415VCQ
CSB-EP745415VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 3-dehydroquinate dehydratase (aroQ) CSB-YP745416VCQ
CSB-EP745416VCQ
CSB-BP745416VCQ
CSB-MP745416VCQ
CSB-EP745416VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP745417VCQ
CSB-EP745417VCQ
CSB-BP745417VCQ
CSB-MP745417VCQ
CSB-EP745417VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus HTH-type transcriptional repressor NsrR (nsrR) CSB-YP745418VCQ
CSB-EP745418VCQ
CSB-BP745418VCQ
CSB-MP745418VCQ
CSB-EP745418VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Protein SlyX homolog (slyX) CSB-YP745419VCQ
CSB-EP745419VCQ
CSB-BP745419VCQ
CSB-MP745419VCQ
CSB-EP745419VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Triosephosphate isomerase (tpiA) CSB-YP745420VCQ
CSB-EP745420VCQ
CSB-BP745420VCQ
CSB-MP745420VCQ
CSB-EP745420VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Acetylornithine deacetylase (argE) CSB-YP745421VCQ
CSB-EP745421VCQ
CSB-BP745421VCQ
CSB-MP745421VCQ
CSB-EP745421VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 30S ribosomal protein S18 (rpsR) CSB-YP745422VCQ
CSB-EP745422VCQ
CSB-BP745422VCQ
CSB-MP745422VCQ
CSB-EP745422VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Methionine synthase (metH), partial CSB-YP745423VCQ
CSB-EP745423VCQ
CSB-BP745423VCQ
CSB-MP745423VCQ
CSB-EP745423VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus UPF0249 protein VV2897 (VV2897) CSB-YP745424VCQ
CSB-EP745424VCQ
CSB-BP745424VCQ
CSB-MP745424VCQ
CSB-EP745424VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus UPF0301 protein VV2869 (VV2869) CSB-YP745425VCQ
CSB-EP745425VCQ
CSB-BP745425VCQ
CSB-MP745425VCQ
CSB-EP745425VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Ribose-5-phosphate isomerase A (rpiA) CSB-YP745426VCQ
CSB-EP745426VCQ
CSB-BP745426VCQ
CSB-MP745426VCQ
CSB-EP745426VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP745427VCQ
CSB-EP745427VCQ
CSB-BP745427VCQ
CSB-MP745427VCQ
CSB-EP745427VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP745428VCQ
CSB-EP745428VCQ
CSB-BP745428VCQ
CSB-MP745428VCQ
CSB-EP745428VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Alanine--tRNA ligase (alaS), partial CSB-YP745429VCQ
CSB-EP745429VCQ
CSB-BP745429VCQ
CSB-MP745429VCQ
CSB-EP745429VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Dephospho-CoA kinase (coaE) CSB-YP745430VCQ
CSB-EP745430VCQ
CSB-BP745430VCQ
CSB-MP745430VCQ
CSB-EP745430VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Iron-sulfur cluster insertion protein ErpA (erpA) CSB-YP745431VCQ
CSB-EP745431VCQ
CSB-BP745431VCQ
CSB-MP745431VCQ
CSB-EP745431VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 30S ribosomal protein S15 (rpsO) CSB-YP745432VCQ
CSB-EP745432VCQ
CSB-BP745432VCQ
CSB-MP745432VCQ
CSB-EP745432VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Galactokinase (galK) CSB-YP745433VCQ
CSB-EP745433VCQ
CSB-BP745433VCQ
CSB-MP745433VCQ
CSB-EP745433VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Na (+)-translocating NADH-quinone reductase subunit E (nqrE), partial CSB-YP745434VCQ1
CSB-EP745434VCQ1
CSB-BP745434VCQ1
CSB-MP745434VCQ1
CSB-EP745434VCQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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