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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Ashbya gossypii Palmitoyltransferase SWF1 (SWF1), partial CSB-YP755092DOT1
CSB-EP755092DOT1
CSB-BP755092DOT1
CSB-MP755092DOT1
CSB-EP755092DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype C Envelope glycoprotein gp160 (env), partial CSB-YP755093HDAH
CSB-EP755093HDAH
CSB-BP755093HDAH
CSB-MP755093HDAH
CSB-EP755093HDAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii U1 small nuclear ribonucleoprotein component SNU71 (SNU71), partial CSB-YP755094DOT
CSB-EP755094DOT
CSB-BP755094DOT
CSB-MP755094DOT
CSB-EP755094DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii 60S ribosomal subunit assembly/export protein LOC1 (LOC1) CSB-YP755095DOT
CSB-EP755095DOT
CSB-BP755095DOT
CSB-MP755095DOT
CSB-EP755095DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii ATPase expression protein 3 (AEP3), partial CSB-YP755096DOT
CSB-EP755096DOT
CSB-BP755096DOT
CSB-MP755096DOT
CSB-EP755096DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Protein ARV1 (ARV1), partial CSB-YP755097DOT1
CSB-EP755097DOT1
CSB-BP755097DOT1
CSB-MP755097DOT1
CSB-EP755097DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Protein STB3 (STB3), partial CSB-YP755098DOT
CSB-EP755098DOT
CSB-BP755098DOT
CSB-MP755098DOT
CSB-EP755098DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Triosephosphate isomerase (TPI1) CSB-YP755099DOT
CSB-EP755099DOT
CSB-BP755099DOT
CSB-MP755099DOT
CSB-EP755099DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mediator of RNA polymerase II transcription subunit 12 (SRB8), partial CSB-YP755100DOT
CSB-EP755100DOT
CSB-BP755100DOT
CSB-MP755100DOT
CSB-EP755100DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Pre-mRNA-splicing factor CWC21 (CWC21) CSB-YP755101DOT
CSB-EP755101DOT
CSB-BP755101DOT
CSB-MP755101DOT
CSB-EP755101DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Superoxide dismutase [Cu-Zn] (SOD1) CSB-YP755102DOT
CSB-EP755102DOT
CSB-BP755102DOT
CSB-MP755102DOT
CSB-EP755102DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Glutamyl-tRNA (Gln) amidotransferase subunit B, mitochondrial (PET112), partial CSB-YP755103DOT
CSB-EP755103DOT
CSB-BP755103DOT
CSB-MP755103DOT
CSB-EP755103DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mitochondrial inner membrane i-AAA protease complex subunit MGR1 (MGR1), partial CSB-YP755104DOT1
CSB-EP755104DOT1
CSB-BP755104DOT1
CSB-MP755104DOT1
CSB-EP755104DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Pre-mRNA-splicing factor CWC23 (CWC23) CSB-YP755105DOT
CSB-EP755105DOT
CSB-BP755105DOT
CSB-MP755105DOT
CSB-EP755105DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Probable transporter MCH1 (MCH1), partial CSB-YP755106DOT
CSB-EP755106DOT
CSB-BP755106DOT
CSB-MP755106DOT
CSB-EP755106DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii GPI inositol-deacylase (BST1), partial CSB-YP755107DOT
CSB-EP755107DOT
CSB-BP755107DOT
CSB-MP755107DOT
CSB-EP755107DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii 60S ribosomal protein L30 (RPL30) CSB-YP755108DOT
CSB-EP755108DOT
CSB-BP755108DOT
CSB-MP755108DOT
CSB-EP755108DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Inosine triphosphate pyrophosphatase (HAM1) CSB-YP755109DOT
CSB-EP755109DOT
CSB-BP755109DOT
CSB-MP755109DOT
CSB-EP755109DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Altered inheritance of mitochondria protein 32 (AIM32) CSB-YP755110DOT
CSB-EP755110DOT
CSB-BP755110DOT
CSB-MP755110DOT
CSB-EP755110DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii DNA replication complex GINS protein SLD5 (SLD5) CSB-YP755111DOT
CSB-EP755111DOT
CSB-BP755111DOT
CSB-MP755111DOT
CSB-EP755111DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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