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重組蛋白

產品名稱 貨號 規格
Recombinant Arabidopsis thaliana F-box/kelch-repeat protein At3g44120 (At3g44120) CSB-YP864884DOA
CSB-EP864884DOA
CSB-BP864884DOA
CSB-MP864884DOA
CSB-EP864884DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At3g58910 (At3g58910) CSB-YP864885DOA
CSB-EP864885DOA
CSB-BP864885DOA
CSB-MP864885DOA
CSB-EP864885DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Histone H3-like 4 (At5g12910) CSB-YP864886DOA
CSB-EP864886DOA
CSB-BP864886DOA
CSB-MP864886DOA
CSB-EP864886DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Nuclear transcription factor Y subunit A-1 (NFYA1) CSB-YP864887DOA
CSB-EP864887DOA
CSB-BP864887DOA
CSB-MP864887DOA
CSB-EP864887DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable pectinesterase 49 (PME49) CSB-YP864888DOA
CSB-EP864888DOA
CSB-BP864888DOA
CSB-MP864888DOA
CSB-EP864888DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At3g56030 (At3g56030) CSB-YP864889DOA
CSB-EP864889DOA
CSB-BP864889DOA
CSB-MP864889DOA
CSB-EP864889DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase RGLG2 (RGLG2) CSB-YP864890DOA
CSB-EP864890DOA
CSB-BP864890DOA
CSB-MP864890DOA
CSB-EP864890DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Mechanosensitive ion channel protein 10 (MSL10), partial CSB-YP864891DOA1
CSB-EP864891DOA1
CSB-BP864891DOA1
CSB-MP864891DOA1
CSB-EP864891DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana PRA1 family protein B1 (PRA1B1), partial CSB-YP864892DOA1
CSB-EP864892DOA1
CSB-BP864892DOA1
CSB-MP864892DOA1
CSB-EP864892DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative two-component response regulator ARR21 (ARR21), partial CSB-YP864893DOA
CSB-EP864893DOA
CSB-BP864893DOA
CSB-MP864893DOA
CSB-EP864893DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Polyamine oxidase 3 (PAO3) CSB-YP864894DOA
CSB-EP864894DOA
CSB-BP864894DOA
CSB-MP864894DOA
CSB-EP864894DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Aspartokinase 1, chloroplastic (AK1) CSB-YP864895DOA
CSB-EP864895DOA
CSB-BP864895DOA
CSB-MP864895DOA
CSB-EP864895DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box/kelch-repeat protein At5g03000 (At5g03000) CSB-YP864896DOA
CSB-EP864896DOA
CSB-BP864896DOA
CSB-MP864896DOA
CSB-EP864896DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana DNA topoisomerase 6 subunit A (TOP6A) CSB-YP864897DOA
CSB-EP864897DOA
CSB-BP864897DOA
CSB-MP864897DOA
CSB-EP864897DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 60S ribosomal protein L35-4 (RPL35D) CSB-YP864898DOA
CSB-EP864898DOA
CSB-BP864898DOA
CSB-MP864898DOA
CSB-EP864898DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable protein phosphatase 2C 66 (PLL2), partial CSB-YP864899DOA
CSB-EP864899DOA
CSB-BP864899DOA
CSB-MP864899DOA
CSB-EP864899DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana GDSL esterase/lipase At5g03980 (At5g03980) CSB-YP864900DOA
CSB-EP864900DOA
CSB-BP864900DOA
CSB-MP864900DOA
CSB-EP864900DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Outer envelope pore protein 16-4, chloroplastic (OEP164), partial CSB-YP864901DOA1
CSB-EP864901DOA1
CSB-BP864901DOA1
CSB-MP864901DOA1
CSB-EP864901DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative two-component response regulator ARR20 (ARR20) CSB-YP864902DOA
CSB-EP864902DOA
CSB-BP864902DOA
CSB-MP864902DOA
CSB-EP864902DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/LRR-repeat protein At3g62440 (At3g62440) CSB-YP864903DOA
CSB-EP864903DOA
CSB-BP864903DOA
CSB-MP864903DOA
CSB-EP864903DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業產品。重組蛋白的技術資源在重組蛋白的開發過程中,除了表達系統之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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