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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana Cellulose synthase-like protein D5 (CSLD5), partial CSB-YP882938DOA
CSB-EP882938DOA
CSB-BP882938DOA
CSB-MP882938DOA
CSB-EP882938DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Peroxiredoxin-2C (PRXIIC) CSB-YP882939DOA
CSB-EP882939DOA
CSB-BP882939DOA
CSB-MP882939DOA
CSB-EP882939DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable calcium-binding protein CML36 (CML36) CSB-YP882940DOA
CSB-EP882940DOA
CSB-BP882940DOA
CSB-MP882940DOA
CSB-EP882940DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pumilio homolog 4 (APUM4), partial CSB-YP882941DOA
CSB-EP882941DOA
CSB-BP882941DOA
CSB-MP882941DOA
CSB-EP882941DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative pentatricopeptide repeat-containing protein At3g01580 (PCMP-E87), partial CSB-YP882942DOA
CSB-EP882942DOA
CSB-BP882942DOA
CSB-MP882942DOA
CSB-EP882942DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor RAP2-12 (RAP2-12) CSB-YP882943DOA
CSB-EP882943DOA
CSB-BP882943DOA
CSB-MP882943DOA
CSB-EP882943DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein LURP-one-related 5 (At1g80120) CSB-YP882944DOA
CSB-EP882944DOA
CSB-BP882944DOA
CSB-MP882944DOA
CSB-EP882944DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At1g74750 (At1g74750), partial CSB-YP882945DOA
CSB-EP882945DOA
CSB-BP882945DOA
CSB-MP882945DOA
CSB-EP882945DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Abscisic acid receptor PYL3 (PYL3) CSB-YP882946DOA
CSB-EP882946DOA
CSB-BP882946DOA
CSB-MP882946DOA
CSB-EP882946DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At1g52620 (At1g52620), partial CSB-YP882947DOA
CSB-EP882947DOA
CSB-BP882947DOA
CSB-MP882947DOA
CSB-EP882947DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Daucus carota Phytoene synthase, chloroplastic (PSY), partial CSB-YP882948DIR
CSB-EP882948DIR
CSB-BP882948DIR
CSB-MP882948DIR
CSB-EP882948DIR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Serpin-Z1C CSB-YP882949TQN
CSB-EP882949TQN
CSB-BP882949TQN
CSB-MP882949TQN
CSB-EP882949TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At4g21300 (PCMP-E36), partial CSB-YP882950DOA
CSB-EP882950DOA
CSB-BP882950DOA
CSB-MP882950DOA
CSB-EP882950DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Receptor like protein kinase S.3 (LECRKS3) CSB-YP882951DOA
CSB-EP882951DOA
CSB-BP882951DOA
CSB-MP882951DOA
CSB-EP882951DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor bHLH126 (BHLH126) CSB-YP882952DOA
CSB-EP882952DOA
CSB-BP882952DOA
CSB-MP882952DOA
CSB-EP882952DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cytochrome P450 71A25 (CYP71A25), partial CSB-YP882953DOA
CSB-EP882953DOA
CSB-BP882953DOA
CSB-MP882953DOA
CSB-EP882953DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana KDEL-tailed cysteine endopeptidase CEP3 (CEP3) CSB-YP882954DOA
CSB-EP882954DOA
CSB-BP882954DOA
CSB-MP882954DOA
CSB-EP882954DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized protein At4g08330, chloroplastic (At4g08330) CSB-YP882955DOA
CSB-EP882955DOA
CSB-BP882955DOA
CSB-MP882955DOA
CSB-EP882955DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 1-aminocyclopropane-1-carboxylate synthase 7 (ACS7) CSB-YP882956DOA
CSB-EP882956DOA
CSB-BP882956DOA
CSB-MP882956DOA
CSB-EP882956DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ABC transporter A family member 6 (ABCA6), partial CSB-YP882957DOA
CSB-EP882957DOA
CSB-BP882957DOA
CSB-MP882957DOA
CSB-EP882957DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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