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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Uncharacterized transporter YcgH (ycgH), partial CSB-YP308655BRJ
CSB-EP308655BRJ
CSB-BP308655BRJ
CSB-MP308655BRJ
CSB-EP308655BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 1-pyrroline-5-carboxylate dehydrogenase 2 (ycgN), partial CSB-YP308656BRJ
CSB-EP308656BRJ
CSB-BP308656BRJ
CSB-MP308656BRJ
CSB-EP308656BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized hydrolase ycgS (ycgS) CSB-YP308657BRJ
CSB-EP308657BRJ
CSB-BP308657BRJ
CSB-MP308657BRJ
CSB-EP308657BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Phenolic acid decarboxylase subunit C (bsdC) CSB-YP308658BRJ
CSB-EP308658BRJ
CSB-BP308658BRJ
CSB-MP308658BRJ
CSB-EP308658BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Sensor histidine kinase yclK (yclK), partial CSB-YP308659BRJ
CSB-EP308659BRJ
CSB-BP308659BRJ
CSB-MP308659BRJ
CSB-EP308659BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized ABC transporter ATP-binding protein YclP (yclP) CSB-YP308660BRJ
CSB-EP308660BRJ
CSB-BP308660BRJ
CSB-MP308660BRJ
CSB-EP308660BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis FMN reductase [NAD (P)H] CSB-YP308661BRJ
CSB-EP308661BRJ
CSB-BP308661BRJ
CSB-MP308661BRJ
CSB-EP308661BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative transport permease yfiN (yfiN), partial CSB-YP308662BRJ1
CSB-EP308662BRJ1
CSB-BP308662BRJ1
CSB-MP308662BRJ1
CSB-EP308662BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pectin lyase (pelB) CSB-YP308663BRI
CSB-EP308663BRI
CSB-BP308663BRI
CSB-MP308663BRI
CSB-EP308663BRI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Peptide deformylase 1 (defA) CSB-YP308664BRJ
CSB-EP308664BRJ
CSB-BP308664BRJ
CSB-MP308664BRJ
CSB-EP308664BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized N-acetyltransferase YnaD (ynaD) CSB-YP308665BRJ
CSB-EP308665BRJ
CSB-BP308665BRJ
CSB-MP308665BRJ
CSB-EP308665BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Protein csk22 (csk22), partial CSB-YP308666BRJ1
CSB-EP308666BRJ1
CSB-BP308666BRJ1
CSB-MP308666BRJ1
CSB-EP308666BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis HTH-type transcriptional regulator gltR (gltR) CSB-YP308667BRJ
CSB-EP308667BRJ
CSB-BP308667BRJ
CSB-MP308667BRJ
CSB-EP308667BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative aminopeptidase ysdC (ysdC) CSB-YP308668BRJ
CSB-EP308668BRJ
CSB-BP308668BRJ
CSB-MP308668BRJ
CSB-EP308668BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Alpha-N-arabinofuranosidase 1 (abfA) CSB-YP308669BRJ
CSB-EP308669BRJ
CSB-BP308669BRJ
CSB-MP308669BRJ
CSB-EP308669BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein YsfB (ysfB) CSB-YP308670BRJ
CSB-EP308670BRJ
CSB-BP308670BRJ
CSB-MP308670BRJ
CSB-EP308670BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Stress response protein ysnF (ysnF) CSB-YP308671BRJ
CSB-EP308671BRJ
CSB-BP308671BRJ
CSB-MP308671BRJ
CSB-EP308671BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ywoF (ywoF) CSB-YP308672BRJ
CSB-EP308672BRJ
CSB-BP308672BRJ
CSB-MP308672BRJ
CSB-EP308672BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP308673BRJ
CSB-EP308673BRJ
CSB-BP308673BRJ
CSB-MP308673BRJ
CSB-EP308673BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative uncharacterized protein ywpD (ywpD) CSB-YP308674BRJ
CSB-EP308674BRJ
CSB-BP308674BRJ
CSB-MP308674BRJ
CSB-EP308674BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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