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重組蛋白

產品名稱 貨號 規格
Recombinant Bacillus pseudofirmus Dipeptide transport system permease protein dppB (dppB), partial CSB-YP310538BRB1
CSB-EP310538BRB1
CSB-BP310538BRB1
CSB-MP310538BRB1
CSB-EP310538BRB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (nodM), partial CSB-YP310539BVW
CSB-EP310539BVW
CSB-BP310539BVW
CSB-MP310539BVW
CSB-EP310539BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP310540DWY
CSB-EP310540DWY
CSB-BP310540DWY
CSB-MP310540DWY
CSB-EP310540DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis ATP-binding/permease protein CydD (cydD), partial CSB-YP310541BRJ1
CSB-EP310541BRJ1
CSB-BP310541BRJ1
CSB-MP310541BRJ1
CSB-EP310541BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized ABC transporter ATP-binding protein YxlF (yxlF) CSB-YP310542BRJ
CSB-EP310542BRJ
CSB-BP310542BRJ
CSB-MP310542BRJ
CSB-EP310542BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized symporter ycgO (ycgO), partial CSB-YP310543BRJ
CSB-EP310543BRJ
CSB-BP310543BRJ
CSB-MP310543BRJ
CSB-EP310543BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0718 protein ycgR (ycgR), partial CSB-YP310544BRJ
CSB-EP310544BRJ
CSB-BP310544BRJ
CSB-MP310544BRJ
CSB-EP310544BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized transcriptional regulatory protein yclJ (yclJ) CSB-YP310545BRJ
CSB-EP310545BRJ
CSB-BP310545BRJ
CSB-MP310545BRJ
CSB-EP310545BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ycnI (ycnI), partial CSB-YP310546BRJ
CSB-EP310546BRJ
CSB-BP310546BRJ
CSB-MP310546BRJ
CSB-EP310546BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Potassium-transporting ATPase A chain (kdpA), partial CSB-YP310547GFM1
CSB-EP310547GFM1
CSB-BP310547GFM1
CSB-MP310547GFM1
CSB-EP310547GFM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Plipastatin synthase subunit D (ppsD), partial CSB-YP310548BRJ
CSB-EP310548BRJ
CSB-BP310548BRJ
CSB-MP310548BRJ
CSB-EP310548BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ynaC (ynaC) CSB-YP310549BRJ
CSB-EP310549BRJ
CSB-BP310549BRJ
CSB-MP310549BRJ
CSB-EP310549BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Branched-chain amino acid transport system carrier protein BrnQ (brnQ), partial CSB-YP310550BRJ
CSB-EP310550BRJ
CSB-BP310550BRJ
CSB-MP310550BRJ
CSB-EP310550BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Antiholin-like protein LrgA (lrgA), partial CSB-YP310551BRJ1
CSB-EP310551BRJ1
CSB-BP310551BRJ1
CSB-MP310551BRJ1
CSB-EP310551BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Sigma-w pathway protein ysdB (ysdB), partial CSB-YP310552BRJ
CSB-EP310552BRJ
CSB-BP310552BRJ
CSB-MP310552BRJ
CSB-EP310552BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Arabinose operon protein AraL (araL) CSB-YP310553BRJ
CSB-EP310553BRJ
CSB-BP310553BRJ
CSB-MP310553BRJ
CSB-EP310553BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis L-arabinose transport system permease protein AraQ (araQ), partial CSB-YP310554BRJ1
CSB-EP310554BRJ1
CSB-BP310554BRJ1
CSB-MP310554BRJ1
CSB-EP310554BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable glycolate oxidase iron-sulfur subunit (glcF) CSB-YP310555BRJ
CSB-EP310555BRJ
CSB-BP310555BRJ
CSB-MP310555BRJ
CSB-EP310555BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized tRNA/rRNA methyltransferase YsgA (ysgA) CSB-YP310556BRJ
CSB-EP310556BRJ
CSB-BP310556BRJ
CSB-MP310556BRJ
CSB-EP310556BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0719 transmembrane protein yshE (yshE), partial CSB-YP310557BRJ1
CSB-EP310557BRJ1
CSB-BP310557BRJ1
CSB-MP310557BRJ1
CSB-EP310557BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業產品。重組蛋白的技術資源在重組蛋白的開發過程中,除了表達系統之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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